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A rapid and highly effective approach for SARS-CoV-2 nucleic acid daily testing in more than four thousand single-tube samples.
Zhou, Yusun; Han, Chunhua; Li, Hui; Zhou, Tingting; Geng, Chao; Luan, Xingwei; Feng, Xiaodong; Sui, Junna; Wang, Shuai; Yu, Teng; Xuan, Chao; Tian, Qingwu.
  • Zhou Y; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Han C; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Li H; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Zhou T; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Geng C; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Luan X; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Feng X; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Sui J; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Wang S; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Yu T; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Xuan C; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
  • Tian Q; Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China.
J Clin Lab Anal ; 36(2): e24211, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1589067
ABSTRACT

BACKGROUND:

Presently, the global spread of COVID-19 is still going on, with more than 0.6 million new cases confirmed per day (as of November 20, 2021). However, since China entered a post-epidemic phase in mid-March 2020, the daily number of new domestic infections in the Chinese mainland has been maintained at almost zero or single digits, which was attributed to a series of effective measures for COVID-19 prevention and control adopted by the Chinese government. Among these measures, SARS-CoV-2 nucleic acid testing holds key role for the timely confirmation and isolation of the infections to prevent further transmission.

METHODS:

Referring to the national policy requirements, since April 30, 2020, The Affiliated Hospital of Qingdao University has conducted SARS-CoV-2 nucleic acid testing in its PCR laboratory for patients and social workers, as well as for environmental monitoring and employee screening. As of mid-November 2020, the daily amount of single-tube samples for nucleic acid testing rose above 4,000.

RESULTS:

In this article, a rapid and highly effective approach for SARS-CoV-2 nucleic acid daily testing is presented, allowing five technicians to complete nucleic acid testing in 6,500 single-tube samples in one day with a high level of quality. Using this approach, since the samples entered the PCR laboratory, all testing results were reported in 2.5-3 h with satisfactory quality control and precise reporting criterion as prerequisites.

CONCLUSION:

This testing approach provides a referable workflow for other testing institutions and is expected to play an important role in COVID-19 prevention and control.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study Topics: Long Covid Limits: Humans Country/Region as subject: Asia Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24211

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acid Amplification Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study Topics: Long Covid Limits: Humans Country/Region as subject: Asia Language: English Journal: J Clin Lab Anal Journal subject: Laboratory Techniques and procedures Year: 2022 Document Type: Article Affiliation country: Jcla.24211