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Detection of N6-methyladenosine in SARS-CoV-2 RNA by methylated RNA immunoprecipitation sequencing.
Li, Na; Rana, Tariq M.
  • Li N; Division of Genetics, Department of Pediatrics, Program in Immunology, University of California San Diego, 9500 Gilman Drive MC 0762, La Jolla, CA 92093, USA.
  • Rana TM; Division of Genetics, Department of Pediatrics, Program in Immunology, University of California San Diego, 9500 Gilman Drive MC 0762, La Jolla, CA 92093, USA.
STAR Protoc ; 3(1): 101067, 2022 03 18.
Article in English | MEDLINE | ID: covidwho-1595326
ABSTRACT
N 6 -methylation of adenosine (m6A) is the most abundant internal mRNA modification and is an important post-transcriptional regulator of gene expression. Here, we describe a protocol for methylated RNA immunoprecipitation sequencing (MeRIP-Seq) to detect and quantify m6A modifications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The protocol is optimized for low viral RNA levels and is readily adaptable for other applications. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenosine / Sequence Analysis, RNA / Immunoprecipitation Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2021.101067

Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenosine / Sequence Analysis, RNA / Immunoprecipitation Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2021.101067