SARS-CoV-2 Variant Screening Using a Virus-Receptor-Based Electrical Biosensor.
Nano Lett
; 22(1): 50-57, 2022 01 12.
Article
in English
| MEDLINE | ID: covidwho-1596792
ABSTRACT
SARS-CoV-2 variants are of particular interest because they can potentially increase the transmissibility and virulence of COVID-19 or reduce the effectiveness of available vaccines. However, screening SARS-CoV-2 variants is a challenge because biosensors target viral components that can mutate. One promising strategy is to screen variants via angiotensin-converting enzyme 2 (ACE2), a virus receptor shared by all known SARS-CoV-2 variants. Here we designed a highly sensitive and portable COVID-19 screening biosensor based on the virus receptor. We chose a dual-gate field-effect transistor to overcome the low sensitivity of virus-receptor-based biosensors. To optimize the biosensor, we introduced a synthetic virus that mimics the important features of SARS-CoV-2 (size, bilayer structure, and composition). The developed biosensor successfully detected SARS-CoV-2 in 20 min and showed sensitivity comparable to that of molecular diagnostic tests (â¼165 copies/mL). Our results indicate that a virus-receptor-based biosensor can be an effective strategy for screening infectious diseases to prevent pandemics.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
Topics:
Vaccines
/
Variants
Limits:
Humans
Language:
English
Journal:
Nano Lett
Year:
2022
Document Type:
Article
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