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A Recent Update on Advanced Molecular Diagnostic Techniques for COVID-19 Pandemic: An Overview.
Roberts, Akanksha; Chouhan, Raghuraj Singh; Shahdeo, Deepshikha; Shrikrishna, Narlawar Sagar; Kesarwani, Veerbhan; Horvat, Milena; Gandhi, Sonu.
  • Roberts A; Department of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
  • Chouhan RS; Department of Environmental Sciences, Jozef Stefan Institute, Ljubljana, Slovenia.
  • Shahdeo D; Department of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
  • Shrikrishna NS; Department of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
  • Kesarwani V; Department of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
  • Horvat M; Department of Environmental Sciences, Jozef Stefan Institute, Ljubljana, Slovenia.
  • Gandhi S; Department of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Front Immunol ; 12: 732756, 2021.
Article in English | MEDLINE | ID: covidwho-1597480
ABSTRACT
Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its related variants on time with precision and accuracy. Currently, the gold standard for detection of SARS-CoV-2 is Reverse Transcription Polymerase Chain Reaction (RT-PCR), but it lacks accuracy, is time-consuming and cumbersome, and fails to detect multi-variant forms of the virus. Herein, we have summarized conventional diagnostic methods such as Chest-CT (Computed Tomography), RT-PCR, Loop Mediated Isothermal Amplification (LAMP), Reverse Transcription-LAMP (RT-LAMP), as well new modern diagnostics such as CRISPR-Cas-based assays, Surface Enhanced Raman Spectroscopy (SERS), Lateral Flow Assays (LFA), Graphene-Field Effect Transistor (GraFET), electrochemical sensors, immunosensors, antisense oligonucleotides (ASOs)-based assays, and microarrays for SARS-CoV-2 detection. This review will also provide an insight into an ongoing research and the possibility of developing more economical tools to tackle the COVID-19 pandemic.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Clinical Laboratory Techniques / Molecular Diagnostic Techniques / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Front Immunol Year: 2021 Document Type: Article Affiliation country: Fimmu.2021.732756

Full text: Available Collection: International databases Database: MEDLINE Main subject: Clinical Laboratory Techniques / Molecular Diagnostic Techniques / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Front Immunol Year: 2021 Document Type: Article Affiliation country: Fimmu.2021.732756