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Column Agglutination Assay Using Polystyrene Microbeads for Rapid Detection of Antibodies against SARS-CoV-2.
Kesarwani, Vidhishri; Walker, Julia A; Henderson, Edward C; Huynh, Gabriel; McLiesh, Heather; Graham, Maryza; Wieringa, Megan; Banaszak Holl, Mark M; Garnier, Gil; Corrie, Simon R.
  • Kesarwani V; Department of Chemical and Biological Engineering, Monash University, Clayton, Victoria 3800, Australia.
  • Walker JA; Bioresource Processing Research Institute of (BioPRIA), Monash University, Clayton, Victoria 3800, Australia.
  • Henderson EC; Centre to Impact AMR, Monash University, Clayton, Victoria 3800, Australia.
  • Huynh G; Department of Chemical and Biological Engineering, Monash University, Clayton, Victoria 3800, Australia.
  • McLiesh H; Bioresource Processing Research Institute of (BioPRIA), Monash University, Clayton, Victoria 3800, Australia.
  • Graham M; Centre to Impact AMR, Monash University, Clayton, Victoria 3800, Australia.
  • Wieringa M; Department of Chemical and Biological Engineering, Monash University, Clayton, Victoria 3800, Australia.
  • Banaszak Holl MM; Bioresource Processing Research Institute of (BioPRIA), Monash University, Clayton, Victoria 3800, Australia.
  • Garnier G; Centre to Impact AMR, Monash University, Clayton, Victoria 3800, Australia.
  • Corrie SR; Department of Chemical and Biological Engineering, Monash University, Clayton, Victoria 3800, Australia.
ACS Appl Mater Interfaces ; 14(2): 2501-2509, 2022 Jan 19.
Article in English | MEDLINE | ID: covidwho-1605760
ABSTRACT
Rapid serology platforms are essential in disease pandemics for a variety of applications, including epidemiological surveillance, contact tracing, vaccination monitoring, and primary diagnosis in resource-limited areas. Laboratory-based enzyme-linked immunosorbent assay (ELISA) platforms are inherently multistep processes that require trained personnel and are of relatively limited throughput. As an alternative, agglutination-based systems have been developed; however, they rely on donor red blood cells and are not yet available for high-throughput screening. Column agglutination tests are a mainstay of pretransfusion blood typing and can be performed at a range of scales, ranging from manual through to fully automated testing. Here, we describe a column agglutination test using colored microbeads coated with recombinant SARS-CoV-2 spike protein that agglutinates when incubated with serum samples collected from patients recently infected with SARS-CoV-2. After confirming specific agglutination, we optimized centrifugal force and time to distinguish samples from uninfected vs SARS-CoV-2-infected individuals and then showed concordant results against ELISA for 22 clinical samples, and also a set of serial bleeds from one donor at days 6-10 postinfection. Our study demonstrates the use of a simple, scalable, and rapid diagnostic platform that can be tailored to detect antibodies raised against SARS-CoV-2 and can be easily integrated with established laboratory frameworks worldwide.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Recombinant Proteins / Agglutination Tests / Diagnostic Tests, Routine / Spike Glycoprotein, Coronavirus / COVID-19 Serological Testing / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.1c17859

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Recombinant Proteins / Agglutination Tests / Diagnostic Tests, Routine / Spike Glycoprotein, Coronavirus / COVID-19 Serological Testing / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2022 Document Type: Article Affiliation country: Acsami.1c17859