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RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).
Lai, Meng Yee; Suppiah, Jeyanthi; Thayan, Ravindran; Ismail, Ilyiana; Mustapa, Nur Izati; Soh, Tuan Suhaila Tuan; Hassan, Afifah Haji; Peariasamy, Kalaiarasu M; Lee, Yee Leng; Lau, Yee Ling.
  • Lai MY; Department of Parasitology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia.
  • Suppiah J; Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Shah Alam, Selangor, Malaysia.
  • Thayan R; Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Shah Alam, Selangor, Malaysia.
  • Ismail I; Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Sungai Buloh, Selangor, Malaysia.
  • Mustapa NI; Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Sungai Buloh, Selangor, Malaysia.
  • Soh TST; Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Sungai Buloh, Selangor, Malaysia.
  • Hassan AH; Department of Pathology, Hospital Sungai Buloh, Ministry of Health, Sungai Buloh, Selangor, Malaysia.
  • Peariasamy KM; Institute for Clinical Research, National Institutes of Health, Ministry of Health, Shah Alam, Selangor, Malaysia.
  • Lee YL; Clinical Research Centre, Hospital Sungai Buloh, Ministry of Health, Sungai Buloh, Selangor, Malaysia.
  • Lau YL; Department of Parasitology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia. lauyeeling@um.edu.my.
Trop Med Health ; 50(1): 2, 2022 Jan 04.
Article in English | MEDLINE | ID: covidwho-1605996
ABSTRACT

BACKGROUND:

Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing.

METHODS:

Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin.

RESULTS:

By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9% sensitivity (95% CI 86 to 99.5%) and 100% specificity (95% CI 78.2-100%).

CONCLUSION:

The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Trop Med Health Year: 2022 Document Type: Article Affiliation country: S41182-021-00396-y

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Trop Med Health Year: 2022 Document Type: Article Affiliation country: S41182-021-00396-y