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Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection.
Wang, Sheng-Hann; Kuo, Chia-Wen; Lo, Shu-Cheng; Yeung, Wing Kiu; Chang, Ting-Wei; Wei, Pei-Kuen.
  • Wang SH; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Kuo CW; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Lo SC; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Yeung WK; Institute of Applied Mechanics, National Taiwan University, Taipei, 11221, Taiwan.
  • Chang TW; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Wei PK; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
J Nanobiotechnology ; 20(1): 6, 2022 Jan 04.
Article in English | MEDLINE | ID: covidwho-1608546
ABSTRACT

BACKGROUND:

Gold nanoparticles (AuNPs) have been widely used in local surface plasmon resonance (LSPR) immunoassays for biomolecule sensing, which is primarily based on two conventional

methods:

absorption spectra analysis and colorimetry. The low figure of merit (FoM) of the LSPR and high-concentration AuNP requirement restrict their limit of detection (LOD), which is approximately ng to µg mL-1 in antibody detection if there is no other signal or analyte amplification. Improvements in sensitivity have been slow in recent for a long time, and pushing the boundary of the current LOD is a great challenge of current LSPR immunoassays in biosensing.

RESULTS:

In this work, we developed spectral image contrast-based flow digital nanoplasmon-metry (Flow DiNM) to push the LOD boundary. Comparing the scattering image brightness of AuNPs in two neighboring wavelength bands near the LSPR peak, the peak shift signal is strongly amplified and quickly detected. Introducing digital analysis, the Flow DiNM provides an ultrahigh signal-to-noise ratio and has a lower sample volume requirement. Compared to the conventional analog LSPR immunoassay, Flow DiNM for anti-BSA detection in pure samples has an LOD as low as 1 pg mL-1 within only a 15-min detection time and 500 µL sample volume. Antibody assays against spike proteins of SARS-CoV-2 in artificial saliva that contained various proteins were also conducted to validate the detection of Flow DiNM in complicated samples. Flow DiNM shows significant discrimination in detection with an LOD of 10 pg mL-1 and a broad dynamic detection range of five orders of magnitude.

CONCLUSION:

Together with the quick readout time and simple operation, this work clearly demonstrated the high sensitivity and selectivity of the developed Flow DiNM in rapid antibody detection. Spectral image contrast and digital analysis further provide a new generation of LSPR immunoassay with AuNPs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Surface Plasmon Resonance / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: J Nanobiotechnology Year: 2022 Document Type: Article Affiliation country: S12951-021-01188-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Surface Plasmon Resonance / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: J Nanobiotechnology Year: 2022 Document Type: Article Affiliation country: S12951-021-01188-6