Your browser doesn't support javascript.
A lethal mouse model for evaluating vaccine-associated enhanced respiratory disease during SARS-CoV-2 infection.
Iwata-Yoshikawa, Naoko; Shiwa, Nozomi; Sekizuka, Tsuyoshi; Sano, Kaori; Ainai, Akira; Hemmi, Takuya; Kataoka, Michiyo; Kuroda, Makoto; Hasegawa, Hideki; Suzuki, Tadaki; Nagata, Noriyo.
  • Iwata-Yoshikawa N; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Shiwa N; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Sekizuka T; Pathogen Genomics Center, National Institute of Infectious Diseases, 162-8640 Tokyo, Japan.
  • Sano K; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Ainai A; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Hemmi T; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Kataoka M; Department of Biological Science and Technology, Tokyo University of Science, 125-8585 Tokyo, Japan.
  • Kuroda M; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Hasegawa H; Pathogen Genomics Center, National Institute of Infectious Diseases, 162-8640 Tokyo, Japan.
  • Suzuki T; Influenza Virus Research Center, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
  • Nagata N; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
Sci Adv ; 8(1): eabh3827, 2022 Jan 07.
Article in English | MEDLINE | ID: covidwho-1612934
ABSTRACT
One safety concern during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine development has been the vaccine-associated enhanced disease, which is characterized by eosinophilic immunopathology and T helper cell type 2 (TH2)­biased immune responses with insufficient neutralizing antibodies. In this study, we established a lethal animal model using BALB/c mice and a mouse-passaged isolate (QHmusX) from a European lineage of SARS-CoV-2. The QHmusX strain induced acute respiratory illness, associated with diffuse alveolar damage and pulmonary edema, in TH2-prone adult BALB/c mice, but not in young mice or TH1-prone C57BL/6 mice. We also showed that immunization of adult BALB/c mice with recombinant spike protein without appropriate adjuvant caused eosinophilic immunopathology with TH2-shifted immune response and insufficient neutralizing antibodies after QHmusX infection. This lethal mouse model is useful for evaluating vaccine-associated enhanced respiratory disease during SARS-CoV-2 infection and may provide new insights into the disease pathogenesis of SARS-CoV-2.

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Topics: Vaccines Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abh3827

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Topics: Vaccines Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abh3827