Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability.

Suarez, Gianmarco D; Suarez, Domenick A; Kiu Tang, Yuki Yu; Zhang, Jing-Xuan; Li, Jun; Nagl, Stefan; Cheung, Peter Pak-Hang

Suarez, Gianmarco D; Suarez, Domenick A; Kiu Tang, Yuki Yu; Zhang, Jing-Xuan; Li, Jun; Nagl, Stefan; Cheung, Peter Pak-Hang.

Suarez GD; Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Suarez DA; Quommni Technologies Limited, Hong Kong.
Kiu Tang YY; Quommni Technologies Limited, Hong Kong.
Zhang JX; Quommni Technologies Limited, Hong Kong.
Li J; Department of Chemistry, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
Nagl S; Department of Chemistry, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
Cheung PP; Department of Chemistry and Key Laboratory of Organic Optoelectronics & Molecular Engineering of Ministry of Education, Tsinghua University, Beijing 100084, China.

Anal Methods ; 14(4): 378-382, 2022 01 27.

Article in English | MEDLINE | ID: covidwho-1617007

ABSTRACT

ABSTRACT

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

Subject(s)

COVID-19; SARS-CoV-2; Colorimetry; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Pandemics; RNA, Viral/genetics; Reproducibility of Results

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Methods Year: 2022 Document Type: Article Affiliation country: D1ay01395e

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