Your browser doesn't support javascript.
Photoswitch-Based Fluorescence Encoding of Microspheres in a Limited Spectral Window for Multiplexed Detection.
Guo, Chao; Zhai, Jingying; Wang, Yifu; Du, Xinfeng; Wang, Zige; Xie, Xiaojiang.
  • Guo C; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
  • Zhai J; Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, Shenzhen 518055, China.
  • Wang Y; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
  • Du X; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
  • Wang Z; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
  • Xie X; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
Anal Chem ; 94(3): 1531-1536, 2022 01 25.
Article in English | MEDLINE | ID: covidwho-1621192
ABSTRACT
Fluorescence barcoding with multicolor fluorophores is limited by spectral crowding. Herein, we propose a fluorescence encoding method in a single-color channel with photoswitches. The photochromic naphthopyran was used to mediate the fluorescence of polystyrene microspheres through resonance energy transfer. The initial fluorescence intensity (F0) and the fluorescence after UV light activation (F/F0) were combined to generate hundreds of 2-dimensional barcodes. The coding capacity was further expanded with the different chemical kinetics of the photoswitches. The photoswitch-based fluorescence barcodes were applied to simultaneously and selectively detect the DNA sequences of COVID-19 (with related mutations) as a proof-of-concept for real applications. The compatibility with the state-of-the-art fluorescence microscopes and simple encoding and decoding make the method very attractive for multiplexed and high-throughput analyses.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Humans Language: English Journal: Anal Chem Year: 2022 Document Type: Article Affiliation country: Acs.analchem.1c04856

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Humans Language: English Journal: Anal Chem Year: 2022 Document Type: Article Affiliation country: Acs.analchem.1c04856