Mini-synplastomes for plastid genetic engineering.
Plant Biotechnol J
; 20(2): 360-373, 2022 02.
Article
in English
| MEDLINE | ID: covidwho-1621953
ABSTRACT
In the age of synthetic biology, plastid engineering requires a nimble platform to introduce novel synthetic circuits in plants. While effective for integrating relatively small constructs into the plastome, plastid engineering via homologous recombination of transgenes is over 30 years old. Here we show the design-build-test of a novel synthetic genome structure that does not disturb the native plastome the 'mini-synplastome'. The mini-synplastome was inspired by dinoflagellate plastome organization, which is comprised of numerous minicircles residing in the plastid instead of a single organellar genome molecule. The first mini-synplastome in plants was developed in vitro to meet the following criteria (i) episomal replication in plastids; (ii) facile cloning; (iii) predictable transgene expression in plastids; (iv) non-integration of vector sequences into the endogenous plastome; and (v) autonomous persistence in the plant over generations in the absence of exogenous selection pressure. Mini-synplastomes are anticipated to revolutionize chloroplast biotechnology, enable facile marker-free plastid engineering, and provide an unparalleled platform for one-step metabolic engineering in plants.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Genetic Engineering
/
Plastids
Type of study:
Prognostic study
Language:
English
Journal:
Plant Biotechnol J
Journal subject:
Biotechnology
/
Botany
Year:
2022
Document Type:
Article
Affiliation country:
Pbi.13717
Similar
MEDLINE
...
LILACS
LIS