Screening and sequencing monoclonal antibody at single-cell level
Optics in Health Care and Biomedical Optics XI 2021
; 11900, 2021.
Article
in English
| Scopus | ID: covidwho-1621985
ABSTRACT
Acquisition of the genes encoding variable regions of paired heavy and light chains (VHVL) is crucial, but it is a labor and cost-intensive process in traditional methods. This study presents a novel method in which all processing steps for acquiring natively paired VHVL genes from single cells are finished in a single microfluidic chip. The microfluidic chip performs single-cell trap/in situ fluorescent examination of antibody specificity/cell lysis/gene amplification all at single-cell level. By a proof-of-concept validation of efficiently acquiring paired VHVL genes of anti-RBD (which is a key protein of SARS-CoV-2 virus) mAbs from single hybridomas, the microfluidic chip has been proved capable of remarkably improving cell loss/human labor/time cost, and more importantly, determinacy of native VHVL genes pairing which is one of the most decisive factors of effectiveness for antibody discovery. © 2021 SPIE.
Full text:
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Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Optics in Health Care and Biomedical Optics XI 2021
Year:
2021
Document Type:
Article
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