Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo.
BMC Res Notes
; 14(1): 461, 2021 Dec 20.
Article
in English
| MEDLINE | ID: covidwho-1629128
ABSTRACT
OBJECTIVE:
Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at - 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidirectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed.RESULTS:
CoV-RNA was detected in ten specimens (47.6%, n = 21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Chiroptera
/
Coronavirus
Type of study:
Observational study
/
Prognostic study
/
Randomized controlled trials
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
BMC Res Notes
Year:
2021
Document Type:
Article
Affiliation country:
S13104-021-05880-6
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