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Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo.
Tan, Cheng-Siang; Noni, Vaenessa; Sathiya Seelan, Jaya Seelan; Denel, Azroie; Anwarali Khan, Faisal Ali.
  • Tan CS; Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia. cstan@unimas.my.
  • Noni V; Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia.
  • Sathiya Seelan JS; Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia.
  • Denel A; Sarawak Forestry Corporation, Kota Sentosa, Sarawak, Malaysia.
  • Anwarali Khan FA; Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia.
BMC Res Notes ; 14(1): 461, 2021 Dec 20.
Article in English | MEDLINE | ID: covidwho-1629128
ABSTRACT

OBJECTIVE:

Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at - 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidirectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed.

RESULTS:

CoV-RNA was detected in ten specimens (47.6%, n = 21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Chiroptera / Coronavirus Type of study: Observational study / Prognostic study / Randomized controlled trials Limits: Animals / Humans Country/Region as subject: Asia Language: English Journal: BMC Res Notes Year: 2021 Document Type: Article Affiliation country: S13104-021-05880-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Chiroptera / Coronavirus Type of study: Observational study / Prognostic study / Randomized controlled trials Limits: Animals / Humans Country/Region as subject: Asia Language: English Journal: BMC Res Notes Year: 2021 Document Type: Article Affiliation country: S13104-021-05880-6