Your browser doesn't support javascript.
Negatively charged residues in the membrane ordering activity of SARS-CoV-1 and -2 fusion peptides.
Lai, Alex L; Freed, Jack H.
  • Lai AL; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York. Electronic address: ll564@cornell.edu.
  • Freed JH; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York. Electronic address: jhf3@cornell.edu.
Biophys J ; 121(2): 207-227, 2022 01 18.
Article in English | MEDLINE | ID: covidwho-1634388
ABSTRACT
Entry of coronaviruses into host cells is mediated by the viral spike protein. Previously, we identified the bona fide fusion peptides (FPs) for severe acute respiratory syndrome coronavirus ("SARS-1") and severe acute respiratory syndrome coronavirus-2 ("SARS-2") using electron spin resonance spectroscopy. We also found that their FPs induce membrane ordering in a Ca2+-dependent fashion. Here we study which negatively charged residues in SARS-1 FP are involved in this binding, to build a topological model and clarify the role of Ca2+. Our systematic mutation study on the SARS-1 FP shows that all six negatively charged residues contribute to the FP's membrane ordering activity, with D812 the dominant residue. The corresponding SARS-2 residue D830 plays an equivalent role. We provide a topological model of how the FP binds Ca2+ ions its two segments FP1 and FP2 each bind one Ca2+. The binding of Ca2+, the folding of FP (both studied by isothermal titration calorimetry experiments), and the ordering activity correlate very well across the mutants, suggesting that the Ca2+ helps the folding of FP in membranes to enhance the ordering activity. Using a novel pseudotyped viral particle-liposome methodology, we monitored the membrane ordering induced by the FPs in the whole spike protein in its trimer form in real time. We found that the SARS-1 and SARS-2 pseudotyped viral particles also induce membrane ordering to the extent that separate FPs do, and mutations of the negatively charged residues also significantly suppress the membrane ordering activity. However, the slower kinetics of the FP ordering activity versus that of the pseudotyped viral particle suggest the need for initial trimerization of the FPs.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Membrane Fusion Limits: Humans Language: English Journal: Biophys J Year: 2022 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Membrane Fusion Limits: Humans Language: English Journal: Biophys J Year: 2022 Document Type: Article