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Construction of SARS-CoV-2 virus-like particles in plant.
Moon, Ki-Beom; Jeon, Jae-Heung; Choi, Hyukjun; Park, Ji-Sun; Park, Su-Jin; Lee, Hyo-Jun; Park, Jeong Mee; Cho, Hye Sun; Moon, Jae Sun; Oh, Hyunwoo; Kang, Sebyung; Mason, Hugh S; Kwon, Suk-Yoon; Kim, Hyun-Soon.
  • Moon KB; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Jeon JH; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Choi H; Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-Gil 50, Ulsan, 44919, Republic of Korea.
  • Park JS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Park SJ; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Lee HJ; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Park JM; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Cho HS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Moon JS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Oh H; Core Facility Management Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Kang S; Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-Gil 50, Ulsan, 44919, Republic of Korea.
  • Mason HS; Center for Immunotherapy, Vaccines, and Virotherapy (CIVV), The Biodesign Institute at ASU, Tempe, AZ, 85287, USA.
  • Kwon SY; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. sykwon@kribb.re.kr.
  • Kim HS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. hyuns@kribb.re.kr.
Sci Rep ; 12(1): 1005, 2022 01 19.
Article in English | MEDLINE | ID: covidwho-1635617
ABSTRACT
The pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused a public health emergency, and research on the development of various types of vaccines is rapidly progressing at an unprecedented development speed internationally. Some vaccines have already been approved for emergency use and are being supplied to people around the world, but there are still many ongoing efforts to create new vaccines. Virus-like particles (VLPs) enable the construction of promising platforms in the field of vaccine development. Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. Plant-derived VLPs were purified by sedimentation through a sucrose cushion. The shape and size of plant-derived VLPs are similar to native SARS-CoV-2 VLPs without spike. Although the assembled VLPs do not have S protein spikes, they could be developed as formulations that can improve the immunogenicity of vaccines including S antigens, and further could be used as platforms that can carry S antigens of concern for various mutations.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / Viroporin Proteins / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines / Coronavirus M Proteins / SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Virus-Like Particle / Viroporin Proteins / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines / Coronavirus M Proteins / SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article