Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing.
PLoS One
; 17(1): e0262170, 2022.
Article
in English
| MEDLINE | ID: covidwho-1637228
ABSTRACT
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly in Spike gene. Monitoring the S gene mutations is crucial for successful controlling measures and detecting variants that can evade vaccine immunity. Even after the costs reduction resulting from the pandemic, the new generation sequencing methodologies remain unavailable to a large number of scientific groups. Therefore, to support the urgent surveillance of SARS-CoV-2 S gene, this work describes a new feasible protocol for complete nucleotide sequencing of the S gene using the Sanger technique. Such a methodology could be easily adopted by any laboratory with experience in sequencing, adding to effective surveillance of SARS-CoV-2 spreading and evolution.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Sequence Analysis, RNA
/
Reverse Transcriptase Polymerase Chain Reaction
/
Pandemics
/
Spike Glycoprotein, Coronavirus
/
Genes, Viral
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Observational study
/
Randomized controlled trials
Topics:
Vaccines
/
Variants
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
PLoS One
Journal subject:
Science
/
Medicine
Year:
2022
Document Type:
Article
Affiliation country:
Journal.pone.0262170
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