Xuanfei Baidu Decoction reduces acute lung injury by regulating infiltration of neutrophils and macrophages via PD-1/IL17A pathway.
Pharmacol Res
; 176: 106083, 2022 02.
Article
in English
| MEDLINE | ID: covidwho-1638968
ABSTRACT
The pathogenic hyper-inflammatory response has been revealed as the major cause of the severity and death of the Corona Virus Disease 2019 (COVID-19). Xuanfei Baidu Decoction (XFBD) as one of the "three medicines and three prescriptions" for the clinically effective treatment of COVID-19 in China, shows unique advantages in the control of symptomatic transition from moderate to severe disease states. However, the roles of XFBD to against hyper-inflammatory response and its mechanism remain unclear. Here, we established acute lung injury (ALI) model induced by lipopolysaccharide (LPS), presenting a hyperinflammatory process to explore the pharmacodynamic effect and molecular mechanism of XFBD on ALI. The in vitro experiments demonstrated that XFBD inhibited the secretion of IL-6 and TNF-α and iNOS activity in LPS-stimulated RAW264.7 macrophages. In vivo, we confirmed that XFBD improved pulmonary injury via down-regulating the expression of proinflammatory cytokines such as IL-6, TNF-α and IL1-ß as well as macrophages and neutrophils infiltration in LPS-induced ALI mice. Mechanically, we revealed that XFBD treated LPS-induced acute lung injury through PD-1/IL17A pathway which regulates the infiltration of neutrophils and macrophages. Additionally, one major compound from XFBD, i.e. glycyrrhizic acid, shows a high binding affinity with IL17A. In conclusion, we demonstrated the therapeutic effects of XFBD, which provides the immune foundations of XFBD and fatherly support its clinical applications.
Keywords
Acteoside (PubChem CID: 5281800); Acute lung injury; Amygdalin (PubChem CID: 656516); COVID-19; Ephedrine (PubChem CID: 9294); Glycyrrhizic acid (PubChem CID: 14982); Hastatoside (PubChem CID: 92043450); Liquiritin (PubChem CID: 503737); Naringin (PubChem CID: 442428); PD-1/IL17A pathway; Polydatin (PubChem CID: 5281718); Sinapine (PubChem CID: 5280385); Systematic network pharmacological analysis; Verbenalin (PubChem CID: 73467); Xuanfei Baidu Decoction
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Drugs, Chinese Herbal
/
Signal Transduction
/
Interleukin-17
/
Acute Lung Injury
/
Programmed Cell Death 1 Receptor
/
Macrophages
/
Neutrophils
Type of study:
Prognostic study
Topics:
Traditional medicine
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Pharmacol Res
Journal subject:
Pharmacology
Year:
2022
Document Type:
Article
Affiliation country:
J.phrs.2022.106083
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