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US FDA-validated TLC method with four greenness assessment evaluations for simultaneous determination of prednisolone and esomeprazole in spiked human plasma.
Abdelhamid, Nessreen S; Magdy, Maimana A; Anwar, Basma H; Farid, Nehal F.
  • Abdelhamid NS; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
  • Magdy MA; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
  • Anwar BH; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
  • Farid NF; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
Biomed Chromatogr ; 36(5): e5343, 2022 May.
Article in English | MEDLINE | ID: covidwho-1640672
ABSTRACT
Recently, prednisolone has been used in treating many medical conditions, such as autoimmune diseases and cancer. It is also prescribed to mitigate the respiratory complications caused by COVID-19 infection. It can cause some health complications, such as GIT ulcers, so it should be co-administered with proton-pump inhibitors, such as esomeprazole, to prevent the risk of ulcers. This work aims to develop an ecofriendly and sensitive TLC method for simultaneous determination of esomeprazole and prednisolone in their binary mixtures and spiked human plasma. Separation was performed using a mixture of ethyl acetate, methanol, and ammonia (9.50.50.1, v/v/v) as an eluting system with UV scanning at 245 nm. Dapoxetine was used as an internal standard to correct the variation during sampling. The resulting Rf values for plasma, esomeprazole, prednisolone, and dapoxetine were 0.03, 0.51, 0.72 and 0.85, respectively. Four greenness assessment tools-national environmental method index, eco-scale assessments, analytical greenness metric approach (AGREE), and green analytical procedure index (GAPI)-were used to evaluate the greenness characteristics of the proposed method to the environment, and the results were acceptable and satisfactory. Validation parameters were checked according to the US FDA guidelines to achieve the international requirements for bioanalytical method validation, and the results were within the accepted ranges.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Esomeprazole / COVID-19 Limits: Humans Language: English Journal: Biomed Chromatogr Year: 2022 Document Type: Article Affiliation country: Bmc.5343

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Esomeprazole / COVID-19 Limits: Humans Language: English Journal: Biomed Chromatogr Year: 2022 Document Type: Article Affiliation country: Bmc.5343