Investigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patients.
Gene Rep
; 26: 101497, 2022 Mar.
Article
in English
| MEDLINE | ID: covidwho-1641268
ABSTRACT
BACKGROUND:
COVID-19 pandemic spread around the world like an infectious disease that presents waved effects on patients. Some patients needed ICU and respiratory support. Some patients only had flu-like symptoms. Cytokine storm and elevated ROS were serious problems for treatment. Apoptotic genes and CYP Family are part of these mechanisms.AIM:
In this study, our aim was to examine the gene expression CYP2E1 and Caspase-3 in patients with COVID-19 infection.METHOD:
60 COVID-19(+) patients (ICU and non-ICU patients) and 30 healthy volunteers were enrolled to study. To measure the level of gene expression qPCR was used. The 2-ΔΔCt method was utilized to analyze gene expression.RESULTS:
The expression of CYP2E1 and Caspase-3 genes showed a significant discrepancy between patients and healthy individuals. Caspase-3 expression increased (p=0,0041) but CYP2E1 expression decreased (p=0,0214) in COVID-19 patients compared to healthy individuals. Both levels of gene expression were lower in patients with affected lungs than patients with unaffected lungs (p<0,05). Laboratory findings including d-Dimer, LDH, platelet count, lymphocyte count were related to both gene expressions (p<0,05). We found no correlation between CYP2E1 and Caspase-3 expressions.CONCLUSION:
The expression of Caspase-3 demonstrated apoptotic situations of patients but was not related to the CYP2E1 expression level. CYP2E1 gene expression is an important actor to metabolize endogens and xenobiotics however, COVID-19 patients demonstrated decreased CYP2E1 expression. CYP2E1 and Caspase-3 gene expression levels may be used as a diagnostic tool for COVID-19 patients.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
Gene Rep
Year:
2022
Document Type:
Article
Affiliation country:
J.genrep.2022.101497
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