Your browser doesn't support javascript.
SARS-CoV-2 RT-qPCR Test Detection Rates Are Associated with Patient Age, Sex, and Time since Diagnosis.
Levine-Tiefenbrun, Matan; Yelin, Idan; Uriel, Hedva; Kuint, Jacob; Schreiber, Licita; Herzel, Esma; Katz, Rachel; Ben-Tov, Amir; Gazit, Sivan; Patalon, Tal; Chodick, Gabriel; Kishony, Roy.
  • Levine-Tiefenbrun M; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel.
  • Yelin I; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel. Electronic address: idany@technion.ac.il.
  • Uriel H; Faculty of Computer Science, Technion-Israel Institute of Technology, Haifa, Israel.
  • Kuint J; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
  • Schreiber L; Maccabi Megalab, Maccabi Healthcare Services, Rehovot, Israel.
  • Herzel E; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
  • Katz R; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
  • Ben-Tov A; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
  • Gazit S; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
  • Patalon T; Maccabitech, Maccabi Health Services, Tel Aviv, Israel.
  • Chodick G; Maccabitech, Maccabi Health Services, Tel Aviv, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
  • Kishony R; Biology Faculty, Technion-Israel Institute of Technology, Haifa, Israel; Faculty of Computer Science, Technion-Israel Institute of Technology, Haifa, Israel.
J Mol Diagn ; 24(2): 112-119, 2022 02.
Article in English | MEDLINE | ID: covidwho-1641441
ABSTRACT
Quantifying the detection rate of the widely used quantitative RT-PCR (RT-qPCR) test for severe acute respiratory syndrome coronavirus 2 and its dependence on patient demographic characteristics and disease progression is key in designing epidemiologic strategies. Analyzing 843,917 test results of 521,696 patients, a "positive period" was defined for each patient between diagnosis of coronavirus disease 2019 and the last positive test result. The fraction of positive test results within this period was then used to estimate detection rate. Regression analyses were used to determine associations of detection with time of sampling after diagnosis, patient demographic characteristics, and viral RNA copy number based on RT-qPCR cycle threshold values of the next positive test result. The overall detection rate in tests performed within 14 days after diagnosis was 83.1%. This rate was higher at days 0 to 5 after diagnosis (89.3%). Furthermore, detection rate was strongly associated with age and sex. Finally, the detection rate with the Allplex 2019-nCoV RT-qPCR kit was associated, at the single-patient level, with viral RNA copy number (P < 10-9). These results show that the reliability of the test result is reduced in later days as well as for women and younger patients, in whom the viral loads are typically lower.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study / Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Mol Diagn Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: J.jmoldx.2021.10.010

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study / Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Mol Diagn Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: J.jmoldx.2021.10.010