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Comprehensive Analysis of CD4+ T Cell Response Cross-Reactive to SARS-CoV-2 Antigens at the Single Allele Level of HLA Class II.
Hyun, You-Seok; Lee, Yong-Hun; Jo, Hyeong-A; Baek, In-Cheol; Kim, Sun-Mi; Sohn, Hyun-Jung; Kim, Tai-Gyu.
  • Hyun YS; Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Lee YH; Department of Biomedicine and Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Jo HA; Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Baek IC; Department of Biomedicine and Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Kim SM; Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Sohn HJ; Department of Biomedicine and Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
  • Kim TG; Catholic Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Front Immunol ; 12: 774491, 2021.
Article in English | MEDLINE | ID: covidwho-1648672
ABSTRACT
Common human coronaviruses have been circulating undiagnosed worldwide. These common human coronaviruses share partial sequence homology with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); therefore, T cells specific to human coronaviruses are also cross-reactive with SARS-CoV-2 antigens. Herein, we defined CD4+ T cell responses that were cross-reactive with SARS-CoV-2 antigens in blood collected in 2016-2018 from healthy donors at the single allele level using artificial antigen-presenting cells (aAPC) expressing a single HLA class II allotype. We assessed the allotype-restricted responses in the 42 individuals using the aAPCs matched 22 HLA-DR alleles, 19 HLA-DQ alleles, and 13 HLA-DP alleles. The response restricted by the HLA-DR locus showed the highest magnitude, and that by HLA-DP locus was higher than that by HLA-DQ locus. Since two alleles of HLA-DR, -DQ, and -DP loci are expressed co-dominantly in an individual, six different HLA class II allotypes can be used to the cross-reactive T cell response. Of the 16 individuals who showed a dominant T cell response, five, one, and ten showed a dominant response by a single allotype of HLA-DR, -DQ, and -DP, respectively. The single allotype-restricted T cells responded to only one antigen in the five individuals and all the spike, membrane, and nucleocapsid proteins in the six individuals. In individuals heterozygous for the HLA-DPA and HLA-DPB loci, four combinations of HLA-DP can be expressed, but only one combination showed a dominant response. These findings demonstrate that cross-reactive T cells to SARS-CoV-2 respond with single-allotype dominance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / HLA-D Antigens / Genes, MHC Class II / Alleles / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Randomized controlled trials Limits: Adult / Female / Humans / Male / Young adult Language: English Journal: Front Immunol Year: 2021 Document Type: Article Affiliation country: Fimmu.2021.774491

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Full text: Available Collection: International databases Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / HLA-D Antigens / Genes, MHC Class II / Alleles / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Randomized controlled trials Limits: Adult / Female / Humans / Male / Young adult Language: English Journal: Front Immunol Year: 2021 Document Type: Article Affiliation country: Fimmu.2021.774491