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Overview of Anti-SARS-CoV-2 Immune Response Six Months after BNT162b2 mRNA Vaccine.
Gandolfo, Claudia; Anichini, Gabriele; Mugnaini, Marco; Bocchia, Monica; Terrosi, Chiara; Sicuranza, Anna; Gori Savellini, Gianni; Gozzetti, Alessandro; Franchi, Federico; Cusi, Maria Grazia.
  • Gandolfo C; Virology Unit, Department of Medical Biotechnologies, University of Siena, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Anichini G; Virology Unit, Department of Medical Biotechnologies, University of Siena, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Mugnaini M; Department of Information Engineering and Mathematical Sciences, University of Siena, 53100 Siena, Italy.
  • Bocchia M; Hematology Unit, Department of Medical Sciences, Surgery and Neurosciences, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Terrosi C; Virology Unit, Department of Medical Biotechnologies, University of Siena, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Sicuranza A; Hematology Unit, Department of Medical Sciences, Surgery and Neurosciences, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Gori Savellini G; Virology Unit, Department of Medical Biotechnologies, University of Siena, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Gozzetti A; Hematology Unit, Department of Medical Sciences, Surgery and Neurosciences, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
  • Franchi F; Anesthesia and Intensive Care Unit, Department of Medicine, Surgery and Neuroscience, University of Siena, 53100 Siena, Italy.
  • Cusi MG; Virology Unit, Department of Medical Biotechnologies, University of Siena, Azienda, Ospedaliera Universitaria Senese, V. le Bracci, 16, 53100 Siena, Italy.
Vaccines (Basel) ; 10(2)2022 Jan 22.
Article in English | MEDLINE | ID: covidwho-1650108
ABSTRACT

BACKGROUND:

We have designed a prospective study aiming to monitor the immune response in 178 health care workers six months after BNT162b2 mRNA vaccination.

METHODS:

The humoral immune response of all subjects was evaluated by chemiluminescence (CMIA); in 60 serum samples, a live virus-based neutralization assay was also tested. Moreover, 6 months after vaccination, B- and T-cell subsets from 20 subjects were observed by FACS analysis after restimulation with the trimeric SARS-CoV-2 Spike protein as an antigen, thus mimicking reinfection in vitro.

RESULTS:

A significant decrease of circulating IgG levels and neutralizing antibodies over time were observed. Moreover, six months after vaccination, a variable T-cell immune response after in vitro antigen stimulation of PBMC was observed. On the contrary, the analysis of B-cell response showed a shift from unswitched to switched memory B-cells and an increase of Th17 cells.

CONCLUSIONS:

Although the variability of the CD4+ and CD8+ immune response and an antibody decline was observed among vaccinated subjects, the increase of switched memory B-cells and Th17 cells, correlating with the presence of neutralizing antibodies, opened the debate on the correct timing of vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Vaccines10020171

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Vaccines10020171