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A rapid water bath PCR combined with lateral flow assay for the simultaneous detection of SARS-CoV-2 and influenza B virus.
Chen, Hong; Wang, Yunxiang; Wei, Hongjuan; Rong, Zhen; Wang, Shengqi.
  • Chen H; Beijing Institute of Radiation Medicine Beijing 100850 P. R. China sqwang@bmi.ac.cn rongzhen0525@sina.com.
  • Wang Y; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases Beijing 100850 P. R. China.
  • Wei H; Beijing Institute of Radiation Medicine Beijing 100850 P. R. China sqwang@bmi.ac.cn rongzhen0525@sina.com.
  • Rong Z; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases Beijing 100850 P. R. China.
  • Wang S; Beijing Institute of Radiation Medicine Beijing 100850 P. R. China sqwang@bmi.ac.cn rongzhen0525@sina.com.
RSC Adv ; 12(6): 3437-3444, 2022 Jan 24.
Article in English | MEDLINE | ID: covidwho-1655682
ABSTRACT
The outbreak of the coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in significant global health and economic threats to the human society. Thus, a rapid and accurate detection method for early testing and diagnosis should be established. In this study, a rapid water bath polymerase chain reaction (PCR) combined with lateral flow assay was developed to detect SARS-CoV-2 and influenza B virus simultaneously. A homemade automated transfer device equipped with reaction tube shuttled rapidly between two water baths at 98 °C and 53 °C to realize rapid PCR. After amplification, two-ended labeled PCR products were detected using the lateral flow strip with two test lines and streptavidin-conjugated quantum dot nanobeads. The fluorescence value was read using a handheld instrument. The established assay could complete reverse-transcription PCR amplification and lateral flow detection in 45 minutes. The detection limits were 8.44 copies per µL and 14.23 copies per µL for SARS-CoV-2 and influenza B virus, respectively. The coefficients of variation of the test strip were 10.10% for the SARS-CoV-2 and 4.94% for the influenza B virus, demonstrating the excellent repeatability of the experiment. These results indicated that the rapid PCR combined with lateral flow assay could detect SARS-CoV-2 and influenza B virus simultaneously at a short assay time and low cost, thereby showing the remarkable potential for the rapid and multiplex detection of respiratory viruses in resource-limited settings.

Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: RSC Adv Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: RSC Adv Year: 2022 Document Type: Article