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Lyophilization provides long-term stability for a lipid nanoparticle-formulated, nucleoside-modified mRNA vaccine.
Muramatsu, Hiromi; Lam, Kieu; Bajusz, Csaba; Laczkó, Dorottya; Karikó, Katalin; Schreiner, Petra; Martin, Alan; Lutwyche, Peter; Heyes, James; Pardi, Norbert.
  • Muramatsu H; Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
  • Lam K; Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada.
  • Bajusz C; Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA; Biotechnological National Laboratory, Institute of Genetics, Biological Research Centre, Szeged 6726, Hungary.
  • Laczkó D; Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
  • Karikó K; Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; BioNTech RNA Pharmaceuticals, Mainz 55131, Germany.
  • Schreiner P; Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada.
  • Martin A; Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada.
  • Lutwyche P; Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada.
  • Heyes J; Genevant Sciences Corporation, Vancouver, BC V5T 4T5, Canada.
  • Pardi N; Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: pnorbert@pennmedicine.upenn.edu.
Mol Ther ; 30(5): 1941-1951, 2022 05 04.
Article in English | MEDLINE | ID: covidwho-1663945
ABSTRACT
Lipid nanoparticle (LNP)-formulated nucleoside-modified mRNA vaccines have proven to be very successful in the fight against the coronavirus disease 2019 (COVID-19) pandemic. They are effective, safe, and can be produced in large quantities. However, the long-term storage of mRNA-LNP vaccines without freezing is still a challenge. Here, we demonstrate that nucleoside-modified mRNA-LNPs can be lyophilized, and the physicochemical properties of the lyophilized material do not significantly change for 12 weeks after storage at room temperature and for at least 24 weeks after storage at 4°C. Importantly, we show in comparative mouse studies that lyophilized firefly luciferase-encoding mRNA-LNPs maintain their high expression, and no decrease in the immunogenicity of a lyophilized influenza virus hemagglutinin-encoding mRNA-LNP vaccine was observed after 12 weeks of storage at room temperature or for at least 24 weeks after storage at 4°C. Our studies offer a potential solution to overcome the long-term storage-related limitations of nucleoside-modified mRNA-LNP vaccines.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza Vaccines / Nanoparticles / COVID-19 Topics: Vaccines Limits: Animals Language: English Journal: Mol Ther Journal subject: Molecular Biology / Therapeutics Year: 2022 Document Type: Article Affiliation country: J.YMTHE.2022.02.001

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza Vaccines / Nanoparticles / COVID-19 Topics: Vaccines Limits: Animals Language: English Journal: Mol Ther Journal subject: Molecular Biology / Therapeutics Year: 2022 Document Type: Article Affiliation country: J.YMTHE.2022.02.001