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Lipidomics and metabolomics signatures of SARS-CoV-2 mediators/receptors in peripheral leukocytes, jejunum and colon.
Mayneris-Perxachs, Jordi; Moreno-Navarrete, José Maria; Ballanti, Marta; Monteleone, Giovanni; Alessandro Paoluzi, Omero; Mingrone, Geltrude; Lefebvre, Philippe; Staels, Bart; Federici, Massimo; Puig, Josep; Garre, Josep; Ramos, Rafael; Fernández-Real, José-Manuel.
  • Mayneris-Perxachs J; Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Girona, Spain.
  • Moreno-Navarrete JM; Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain.
  • Ballanti M; Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.
  • Monteleone G; Girona Biomedical Research Institute (IDIBGI), Dr. Josep Trueta University Hospital, Catalonia, Spain.
  • Alessandro Paoluzi O; Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Girona, Spain.
  • Mingrone G; Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain.
  • Lefebvre P; Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.
  • Staels B; Department of Medical Sciences, School of Medicine, University of Girona, Girona, Spain.
  • Federici M; Girona Biomedical Research Institute (IDIBGI), Dr. Josep Trueta University Hospital, Catalonia, Spain.
  • Puig J; Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
  • Garre J; Center for Atherosclerosis, Policlinico Tor Vergata, Rome, Italy.
  • Ramos R; Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
  • Fernández-Real JM; Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
Comput Struct Biotechnol J ; 19: 6080-6089, 2021.
Article in English | MEDLINE | ID: covidwho-1664834
ABSTRACT
Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. However, emerging research has identified novel mediators such as DPP9, TYK2, and CCR2, all playing a critical role in inflammation. We evaluated the expression of SARS-CoV-2 receptors in peripheral leukocytes (n = 469), jejunum (n = 30), and colon (n = 37) of three independent cohorts by real-time PCR, RNA-sequencing, and microarray transcriptomics. We also performed HPCL-MS/MS lipidomics and metabolomics analyses to identify signatures linked to SARS-CoV-2 receptors. We found markedly higher peripheral leukocytes ACE2 expression levels in women compared to men, whereas the intestinal expression of TMPRSS2 was positively associated with BMI. Consistent lipidomics signatures associated with the expression of these mediators were found in both tissues and peripheral leukocytes involving n-3 long-chain PUFAs and arachidonic acid-derived eicosanoids, which play a key role in the regulation of inflammation and may interfere with viral entry and replication. Medium- and long-chain hydroxy acids, which have shown to interfere in viral replication, were also liked to SARS-CoV2 receptors. Gonadal steroids were also associated with the expression of some of these receptors, even after controlling for sex. The expression of SARS-CoV2 receptors was associated with several metabolic and nutritional traits in different cell types. This information may be useful in the design of potential therapies targeted at coronavirus entry.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Language: English Journal: Comput Struct Biotechnol J Year: 2021 Document Type: Article Affiliation country: J.csbj.2021.11.007

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Language: English Journal: Comput Struct Biotechnol J Year: 2021 Document Type: Article Affiliation country: J.csbj.2021.11.007