Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples.
STAR Protoc
; 3(1): 101177, 2022 03 18.
Article
in English
| MEDLINE | ID: covidwho-1665545
ABSTRACT
With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics. For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Specimen Handling
/
Viral Proteins
/
Nasopharynx
/
Proteomics
/
Tandem Mass Spectrometry
/
SARS-CoV-2
/
COVID-19
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
STAR Protoc
Year:
2022
Document Type:
Article
Affiliation country:
J.xpro.2022.101177
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