Multiplex Digital Polymerase Chain Reaction Technology and Its Application
Chinese Journal of Analytical Chemistry
; 50(1):25-38, 2022.
Article
in Chinese
| Web of Science | ID: covidwho-1667872
ABSTRACT
Digital polymerase chain reaction (PCR), as a nucleic acid detection technology with wide application prospect, has become one of the most accurate nucleic acid detection technology at present. Multiplex detection is an important direction for the development of digital PCR technique. With the development of microfluidic technology, multiplex digital PCR technique has become more and more mature. This paper reviewed the research progresses of multiplex digital PCR in recent years, especially summarized the implementation of multiplex digital PCR technique in the past five years, and introduced the application of multiplex digital PCR technique in hot areas such as liquid biopsy, transgenic detection, and SARS-Cov-2 detection. Finally, the issues and challenges faced by multiplex digital PCR technique were discussed and the future direction of the technology was foreseen.
Full text:
Available
Collection:
Databases of international organizations
Database:
Web of Science
Language:
Chinese
Journal:
Chinese Journal of Analytical Chemistry
Year:
2022
Document Type:
Article
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