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Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potential.
Rodríguez-Enríquez, Alan; Herrera-Camacho, Irma; Millán-Pérez-Peña, Lourdes; Reyes-Leyva, Julio; Santos-López, Gerardo; Rivera-Benítez, José Francisco; Rosas-Murrieta, Nora Hilda.
  • Rodríguez-Enríquez A; Laboratory of Biochemistry and Molecular Biology, Chemistry Center, Institute of Science., Benemérita Universidad Autónoma de Puebla, Puebla, México.
  • Herrera-Camacho I; Postgraduate in Chemical Sciences, Benemérita Universidad Autónoma de Puebla, Puebla, México.
  • Millán-Pérez-Peña L; Laboratory of Biochemistry and Molecular Biology, Chemistry Center, Institute of Science., Benemérita Universidad Autónoma de Puebla, Puebla, México.
  • Reyes-Leyva J; Laboratory of Biochemistry and Molecular Biology, Chemistry Center, Institute of Science., Benemérita Universidad Autónoma de Puebla, Puebla, México.
  • Santos-López G; Molecular Biology and Virology Laboratory, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Metepec, México, México.
  • Rivera-Benítez JF; Molecular Biology and Virology Laboratory, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social (IMSS), Metepec, México, México.
  • Rosas-Murrieta NH; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Ciudad de México, México.
PLoS One ; 17(2): e0263582, 2022.
Article in English | MEDLINE | ID: covidwho-1910522
ABSTRACT
The membrane protein M of the Porcine Epidemic Diarrhea Virus (PEDV) is the most abundant component of the viral envelope. The M protein plays a central role in the morphogenesis and assembly of the virus through protein interactions of the M-M, M-Spike (S) and M-nucleocapsid (N) type. The M protein is known to induce protective antibodies in pigs and to participate in the antagonistic response of the cellular antiviral system coordinated by the type I and type III interferon pathways. The 3D structure of the PEDV M protein is still unknown. The present work exposes a predicted 3D model of the M protein generated using the Robetta protocol. The M protein model is organized into a transmembrane and a globular region. The obtained 3D model of the PEDV M protein was compared with 3D models of the SARS-CoV-2 M protein created using neural networks and with initial machine learning-based models created using trRosetta. The 3D model of the present study predicted four linear B-cell epitopes (RSVNASSGTG and KHGDYSAVSNPSALT peptides are noteworthy), six discontinuous B-cell epitopes, forty weak binding and fourteen strong binding T-cell epitopes in the CV777 M protein. A high degree of conservation of the epitopes predicted in the PEDV M protein was observed among different PEDV strains isolated in different countries. The data suggest that the M protein could be a potential candidate for the development of new treatments or strategies that activate protective cellular mechanisms against viral diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine / Swine Diseases / Coronavirus Infections / Porcine epidemic diarrhea virus / Coronavirus M Proteins Type of study: Prognostic study Limits: Animals Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine / Swine Diseases / Coronavirus Infections / Porcine epidemic diarrhea virus / Coronavirus M Proteins Type of study: Prognostic study Limits: Animals Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article