3D‐Printed SARS‐CoV‐2 RNA Genosensing Microfluidic System

ABSTRACT

Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab‐on‐a‐chip device through electrochemical detection using 3D printing technology for SARS‐CoV‐2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D‐printed by a 3D printing pen (3D‐PP). The 3D‐PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS‐CoV‐2. The sensing mechanism relies on the electro‐oxidation of adenines present in ssDNA when in contact with SARS‐CoV‐2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS‐CoV‐2/3D‐PP genosensor shows high sensitivity and fast response. The strengths of a lab‐on‐a‐chip device and 3D printing technology to fabricate a point‐of‐care to detect SARS‐CoV‐2RNA are combined. The sensing mechanism relies on the electro‐oxidation of adenines present in ssDNA immobilized on 3D printing pen genosensor. In presence of SARS‐CoV‐2 RNA, ssDNA hybridizes and desorbs from the genosensor surface, causing a decrease of the sensor signal.