Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre-analytical conditions.
Alzheimers Dement (Amst)
; 13(1): e12145, 2021.
Article
in English
| MEDLINE | ID: covidwho-1680307
ABSTRACT
INTRODUCTION:
This study investigated alternative pre-analytical handling of blood for neurofilament light (NfL) analysis where resources are limited.METHOD:
Plasma NfL was measured with single molecule array after alternative blood processing procedures dried plasma spots (DPS), dried blood spots (DBS), and delayed 48-hour centrifugation. These were compared to standardized plasma processing (reference standard [RS]). In a discovery cohort (n = 10) and a confirmatory cohort (n = 21), whole blood was obtained from individuals with unknown clinical etiology. In the confirmatory cohort, delayed centrifugation protocol was paired with either 37°C incubation or sample shaking to test the effect of these parameters.RESULTS:
Delayed centrifugation (R2 = 0.991) and DPS (discovery cohort, R2 = 0.954; confirmatory cohort, DPS R2 = 0.961) methods were strongly associated with the RS. Delayed centrifugation with higher temperatures (R2 = 0.995) and shaking (R2 = 0.975) did not affect this association. DPS (P < 0.001) returned concentrations considerably lower than the RS.DISCUSSION:
DPS or delayed centrifugation are viable pre-analytical procedures for the accurate quantification of plasma NfL.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Type of study:
Cohort study
/
Etiology study
/
Observational study
/
Prognostic study
Language:
English
Journal:
Alzheimers Dement (Amst)
Year:
2021
Document Type:
Article
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