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Multiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts.
Dobaño, Carlota; Alonso, Selena; Vidal, Marta; Jiménez, Alfons; Rubio, Rocío; Santano, Rebeca; Barrios, Diana; Pons Tomas, Gemma; Melé Casas, María; Hernández García, María; Girona-Alarcón, Mònica; Puyol, Laura; Baro, Barbara; Millat-Martínez, Pere; Ajanovic, Sara; Balanza, Núria; Arias, Sara; Rodrigo Melero, Natalia; Carolis, Carlo; García-Miquel, Aleix; Bonet-Carné, Elisenda; Claverol, Joana; Cubells, Marta; Fortuny, Claudia; Fumadó, Victoria; Codina, Anna; Bassat, Quique; Muñoz-Almagro, Carmen; Fernández de Sevilla, Mariona; Gratacós, Eduard; Izquierdo, Luis; García-García, Juan José; Aguilar, Ruth; Jordan, Iolanda; Moncunill, Gemma.
  • Dobaño C; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Alonso S; Consorcio de Investigación Biomédica en Red (CIBER) de Enfermedades Infecciosas, Madrid, Spain.
  • Vidal M; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Jiménez A; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Rubio R; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Santano R; Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
  • Barrios D; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Pons Tomas G; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Melé Casas M; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Hernández García M; Pediatrics Department, Hospital Sant Joan de Déu, Universitat de Barcelona, Esplugues, Spain.
  • Girona-Alarcón M; Pediatrics Department, Hospital Sant Joan de Déu, Universitat de Barcelona, Esplugues, Spain.
  • Puyol L; Pediatrics Department, Hospital Sant Joan de Déu, Universitat de Barcelona, Esplugues, Spain.
  • Baro B; Institut de Recerca Sant Joan de Déu, Esplugues, Spain.
  • Millat-Martínez P; Paediatric Intensive Care Unit, Hospital Sant Joan de Déu, Universitat de Barcelona, Barcelona, Spain.
  • Ajanovic S; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Balanza N; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Arias S; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Rodrigo Melero N; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Carolis C; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • García-Miquel A; ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
  • Bonet-Carné E; Biomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
  • Claverol J; Biomolecular Screening and Protein Technologies Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
  • Cubells M; Fetal Medicine Research Center (Hospital Clínic and Hospital Sant Joan de Déu), Universitat de Barcelona, Barcelona, Spain.
  • Fortuny C; Fetal Medicine Research Center (Hospital Clínic and Hospital Sant Joan de Déu), Universitat de Barcelona, Barcelona, Spain.
  • Fumadó V; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
  • Codina A; Universitat Politècnica de Catalunya, BarcelonaTech, Barcelona, Spain.
  • Bassat Q; Institut de Recerca Sant Joan de Déu, Esplugues, Spain.
  • Muñoz-Almagro C; Fundació Sant Joan de Déu, Barcelona, Spain.
  • Fernández de Sevilla M; Institut de Recerca Sant Joan de Déu, Esplugues, Spain.
  • Gratacós E; Fundació Sant Joan de Déu, Barcelona, Spain.
  • Izquierdo L; Institut de Recerca Sant Joan de Déu, Esplugues, Spain.
  • García-García JJ; Infectious Diseases Department, Hospital Sant Joan de Déu, Barcelona, Spain.
  • Aguilar R; Institut de Recerca Sant Joan de Déu, Esplugues, Spain.
  • Jordan I; Infectious Diseases Department, Hospital Sant Joan de Déu, Barcelona, Spain.
  • Moncunill G; Biobank Hospital Sant Joan de Déu, Barcelona, Spain.
Front Immunol ; 13: 751705, 2022.
Article in English | MEDLINE | ID: covidwho-1686480
ABSTRACT
COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently tested negative over the same follow up period (n=60), in the Summer of 2020 in Barcelona, Spain. Saliva antibody levels in SARS-CoV-2 RT-PCR positive individuals were significantly higher than in negative individuals and correlated with those measured in sera/plasmas. Asymptomatic infected individuals had higher levels of anti-S IgG than symptomatic individuals, suggesting a protective anti-disease role for antibodies. Higher anti-S IgG and IgM levels in serum/plasma and saliva, respectively, in infected children compared to infected adults could also be related to stronger clinical immunity in them. Among infected children, males had higher levels of saliva IgG to N and RBD than females. Despite overall correlation, individual clustering analysis suggested that responses that may not be detected in blood could be patent in saliva, and vice versa. In conclusion, measurement of SARS-CoV-2-specific saliva antibodies should be considered as a complementary non-invasive assay to serum/plasma to determine COVID-19 prevalence and transmission in pediatric populations before and after vaccination campaigns.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Saliva / Immunoassay / COVID-19 Serological Testing / COVID-19 / Antibodies, Viral Type of study: Observational study / Risk factors Topics: Vaccines Limits: Adult / Child / Female / Humans / Male Country/Region as subject: Europa Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.751705

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Saliva / Immunoassay / COVID-19 Serological Testing / COVID-19 / Antibodies, Viral Type of study: Observational study / Risk factors Topics: Vaccines Limits: Adult / Child / Female / Humans / Male Country/Region as subject: Europa Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.751705