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Exploring Mitochondrial Localization of SARS-CoV-2 RNA by Padlock Assay: A Pilot Study in Human Placenta.
Gabanella, Francesca; Barbato, Christian; Corbi, Nicoletta; Fiore, Marco; Petrella, Carla; de Vincentiis, Marco; Greco, Antonio; Ferraguti, Giampiero; Corsi, Alessandro; Ralli, Massimo; Pecorella, Irene; Di Gioia, Cira; Pecorini, Francesco; Brunelli, Roberto; Passananti, Claudio; Minni, Antonio; Di Certo, Maria Grazia.
  • Gabanella F; CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Barbato C; CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, Italy.
  • Corbi N; CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Fiore M; CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, Italy.
  • Petrella C; CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • de Vincentiis M; CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Greco A; Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Ferraguti G; Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Corsi A; Department of Experimental Medicine, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Ralli M; Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.
  • Pecorella I; Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Di Gioia C; Department of Radiological, Oncological, and Pathological Sciences, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Pecorini F; Department of Radiological, Oncological, and Pathological Sciences, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Brunelli R; Department of Maternal and Child Health and Urological Sciences, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Passananti C; Department of Maternal and Child Health and Urological Sciences, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
  • Minni A; CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, Italy.
  • Di Certo MG; Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.
Int J Mol Sci ; 23(4)2022 Feb 14.
Article in English | MEDLINE | ID: covidwho-1686821
ABSTRACT
The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal-fetal interface.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Placenta / RNA, Viral / Nucleic Acid Amplification Techniques / SARS-CoV-2 / Mitochondria Limits: Adult / Female / Humans / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23042100

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Placenta / RNA, Viral / Nucleic Acid Amplification Techniques / SARS-CoV-2 / Mitochondria Limits: Adult / Female / Humans / Pregnancy Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23042100