Effect of signal molecules for enhanced production of boeravinone B in shoot cultures of Boerhaavia diffusa (L.)
Emirates Journal of Food and Agriculture
; 33(10):893-898, 2021.
Article
in English
| ProQuest Central | ID: covidwho-1687467
ABSTRACT
High performance thin layer chromatography (HPTLC) analysis was used to analyze boeravinone B production in shoot cultures of Boerhaavia diffusa under the influence of different biotic [yeast extract (YE), cellulase (CL)] and abiotic [salicylic acid (SA), jasmonic acid (JA)] signal molecules at different concentrations. Biomass accumulation and boeravinone B production in shoot cultures raised on agar solidified medium were analysed for a period of 30 days to optimize the suitable age of culture for treatment with signal molecules. A maximum yield of boeravinone B (5.74 %) was obtained after 7 days and therefore treatments were performed at a gap of 3, 6 and 9 days. Signal molecules used at varied concentrations differentially influenced the shoot cultures for biomass regeneration and culture growth. Cellulase treatment (0.5 mgl-1) resulted in maximizing biomass (1.30gm) and boeravinone B content (22.7 %) after 6 days of exposure time as compared to other treatments used in the study. Thus the current study can be exploited further for enhancement of boeravinone B from shoot cultures of Boerhaavia diffusa.
Agriculture; Thin layer chromatography; Acids; Jasmonic acid; Severe acute respiratory syndrome coronavirus 2; Yeasts; Phytochemicals; Cell culture; Biomass; Cellulase; Chromatography; Salicylic acid; Influence; Regeneration; Age; Optimization; Pore size; Metabolites; Variance analysis; United States--US; Boerhavia diffusa
Full text:
Available
Collection:
Databases of international organizations
Database:
ProQuest Central
Type of study:
Experimental Studies
Language:
English
Journal:
Emirates Journal of Food and Agriculture
Year:
2021
Document Type:
Article
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