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Loss of Detection of sgN Precedes Viral Abridged Replication in COVID-19-Affected Patients-A Target for SARS-CoV-2 Propagation.
Ferrucci, Veronica; de Antonellis, Pasqualino; Quarantelli, Fabrizio; Asadzadeh, Fatemeh; Bibbò, Francesca; Siciliano, Roberto; Sorice, Carmen; Pisano, Ida; Izzo, Barbara; Di Domenico, Carmela; Boccia, Angelo; Vargas, Maria; Pierri, Biancamaria; Viscardi, Maurizio; Brandi, Sergio; Fusco, Giovanna; Cerino, Pellegrino; De Pietro, Livia; Furfaro, Ciro; Napolitano, Leonardo Antonio; Paolella, Giovanni; Festa, Lidia; Marzinotto, Stefania; Conte, Maria Concetta; Gentile, Ivan; Servillo, Giuseppe; Curcio, Francesco; de Cristofaro, Tiziana; Broccolo, Francesco; Capoluongo, Ettore; Zollo, Massimo.
  • Ferrucci V; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • de Antonellis P; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Quarantelli F; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Asadzadeh F; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Bibbò F; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Siciliano R; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Sorice C; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Pisano I; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Izzo B; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Di Domenico C; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Boccia A; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Vargas M; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Pierri B; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Viscardi M; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Brandi S; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Fusco G; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • Cerino P; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • De Pietro L; Department of Neurosciences, Reproductive Sciences and Odontostomatology, University of Naples Federico II, 80131 Naples, Italy.
  • Furfaro C; Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Naples, Italy.
  • Napolitano LA; Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Naples, Italy.
  • Paolella G; Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Naples, Italy.
  • Festa L; Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Naples, Italy.
  • Marzinotto S; Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Naples, Italy.
  • Conte MC; U.O.C. Pneumologia, Ospedale Boscotrecase-Asl Napoli3 Sud, 80042 Naples, Italy.
  • Gentile I; Dipartimento di Medicina di Laboratorio e Anatomia Patologica, Nola Hospital, Asl Napoli 3 Sud, 80035 Naples, Italy.
  • Servillo G; Dipartimento di Medicina di Laboratorio e Anatomia Patologica, Nola Hospital, Asl Napoli 3 Sud, 80035 Naples, Italy.
  • Curcio F; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy.
  • de Cristofaro T; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples Federico II, 80131 Naples, Italy.
  • Broccolo F; Unità Speciale di Continuità Assistenziale (USCA), Asl Benevento, 82100 Benevento, Italy.
  • Capoluongo E; Dipartimento di Medicina Clinica e Chirurgia, University of Naples Federico II, 80131 Naples, Italy.
  • Zollo M; Dipartimento di Medicina di Laboratorio, Università degli Studi di Udine, 33100 Udine, Italy.
Int J Mol Sci ; 23(4)2022 Feb 09.
Article in English | MEDLINE | ID: covidwho-1690219
ABSTRACT
The development of prophylactic agents against the SARS-CoV-2 virus is a public health priority in the search for new surrogate markers of active virus replication. Early detection markers are needed to follow disease progression and foresee patient negativization. Subgenomic RNA transcripts (with a focus on sgN) were evaluated in oro/nasopharyngeal swabs from COVID-19-affected patients with an analysis of 315 positive samples using qPCR technology. Cut-off Cq values for sgN (Cq < 33.15) and sgE (Cq < 34.06) showed correlations to high viral loads. The specific loss of sgN in home-isolated and hospitalized COVID-19-positive patients indicated negativization of patient condition, 3-7 days from the first swab, respectively. A new detection kit for sgN, gene E, gene ORF1ab, and gene RNAse P was developed recently. In addition, in vitro studies have shown that 2'-O-methyl antisense RNA (related to the sgN sequence) can impair SARS-CoV-2 N protein synthesis, viral replication, and syncytia formation in human cells (i.e., HEK-293T cells overexpressing ACE2) upon infection with VOC Alpha (B.1.1.7)-SARS-CoV-2 variant, defining the use that this procedure might have for future therapeutic actions against SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virus Replication / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23041941

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virus Replication / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23041941