Your browser doesn't support javascript.
Children and Adults With Mild COVID-19: Dynamics of the Memory T Cell Response up to 10 Months.
Kaaijk, Patricia; Pimentel, Verónica Olivo; Emmelot, Maarten E; Poelen, Martien C. M.; Cevirgel, Alper; Schepp, Rutger M; den Hartog, Gerco; Reukers, Daphne F M; Beckers, Lisa; van Beek, Josine; van Els, Cécile A C M; Meijer, Adam; Rots, Nynke Y; de Wit, Jelle.
  • Kaaijk P; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Pimentel VO; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Emmelot ME; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Poelen MCM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Cevirgel A; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Schepp RM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • den Hartog G; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Reukers DFM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Beckers L; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • van Beek J; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • van Els CACM; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • Meijer A; Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.
  • Rots NY; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
  • de Wit J; Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.
Front Immunol ; 13: 817876, 2022.
Article in English | MEDLINE | ID: covidwho-1902979
ABSTRACT

Background:

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to considerable morbidity/mortality worldwide, but most infections, especially among children, have a mild course. However, it remains largely unknown whether infected children develop cellular immune memory.

Methods:

To determine whether a memory T cell response is being developed, we performed a longitudinal assessment of the SARS-CoV-2-specific T cell response by IFN-γ ELISPOT and activation marker analyses of peripheral blood samples from unvaccinated children and adults with mild-to-moderate COVID-19.

Results:

Upon stimulation of PBMCs with heat-inactivated SARS-CoV-2 or overlapping peptides of spike (S-SARS-CoV-2) and nucleocapsid proteins, we found S-SARS-CoV-2-specific IFN-γ T cell responses in infected children (83%) and adults (100%) that were absent in unexposed controls. Frequencies of SARS-CoV-2-specific T cells were higher in infected adults, especially several cases with moderate symptoms, compared to infected children. The S-SARS-CoV-2 IFN-γ T cell response correlated with S1-SARS-CoV-2-specific serum antibody concentrations. Predominantly, effector memory CD4+ T cells of a Th1 phenotype were activated upon exposure to SARS-CoV-2 antigens. Frequencies of SARS-CoV-2-specific T cells were significantly reduced at 10 months after symptom onset, while S1-SARS-CoV-2-specific IgG concentrations were still detectable in 90% of all children and adults.

Conclusions:

Our data indicate that an antigen-specific T cell and antibody response is developed after mild SARS-CoV-2 infection in children and adults. It remains to be elucidated to what extent this SARS-CoV-2-specific response can contribute to an effective recall response after reinfection.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Th1 Cells / SARS-CoV-2 / COVID-19 / Memory T Cells / Immunologic Memory Type of study: Cohort study / Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.817876

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Th1 Cells / SARS-CoV-2 / COVID-19 / Memory T Cells / Immunologic Memory Type of study: Cohort study / Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.817876