SARS-CoV-2 detection in bioaerosols using a liquid impinger collector and ddPCR.
Indoor Air
; 32(2): e13002, 2022 02.
Article
in English
| MEDLINE | ID: covidwho-1700268
ABSTRACT
The airborne route is the dominant form of COVID-19 transmission, and therefore, the development of methodologies to quantify SARS-CoV-2 in bioaerosols is needed. We aimed to identify SARS-CoV-2 in bioaerosols by using a highly efficient sampler for the collection of 1-3 µm particles, followed by a highly sensitive detection method. 65 bioaerosol samples were collected in hospital rooms in the presence of a COVID-19 patient using a liquid impinger sampler. The SARS-CoV-2 genome was detected by ddPCR using different primer/probe sets. 44.6% of the samples resulted positive for SARS-CoV-2 following this protocol. By increasing the sampled air volume from 339 to 650 L, the percentage of positive samples went from 41% to 50%. We detected five times less positives with a commercial one-step RT-PCR assay. However, the selection of primer/probe sets might be one of the most determining factor for bioaerosol SARS-CoV-2 detection since with the ORF1ab set more than 40% of the samples were positive, compared to <10% with other sets. In conclusion, the use of a liquid impinger collector and ddPCR is an adequate strategy to detect SARS-CoV-2 in bioaerosols. However, there are still some methodological aspects that must be adjusted to optimize and standardize a definitive protocol.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Air Pollution, Indoor
/
SARS-CoV-2
/
COVID-19
/
Respiratory Aerosols and Droplets
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Indoor Air
Journal subject:
Environmental Health
Year:
2022
Document Type:
Article
Affiliation country:
Ina.13002
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