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Secondary Structure of Influenza A Virus Genomic Segment 8 RNA Folded in a Cellular Environment.
Szutkowska, Barbara; Wieczorek, Klaudia; Kierzek, Ryszard; Zmora, Pawel; Peterson, Jake M; Moss, Walter N; Mathews, David H; Kierzek, Elzbieta.
  • Szutkowska B; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
  • Wieczorek K; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
  • Kierzek R; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
  • Zmora P; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
  • Peterson JM; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
  • Moss WN; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
  • Mathews DH; Department of Biochemistry & Biophysics and Center for RNA Biology, 601 Elmwood Avenue, Box 712, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA.
  • Kierzek E; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Int J Mol Sci ; 23(5)2022 Feb 23.
Article in English | MEDLINE | ID: covidwho-1700574
ABSTRACT
Influenza A virus (IAV) is a member of the single-stranded RNA (ssRNA) family of viruses. The most recent global pandemic caused by the SARS-CoV-2 virus has shown the major threat that RNA viruses can pose to humanity. In comparison, influenza has an even higher pandemic potential as a result of its high rate of mutations within its relatively short (<13 kbp) genome, as well as its capability to undergo genetic reassortment. In light of this threat, and the fact that RNA structure is connected to a broad range of known biological functions, deeper investigation of viral RNA (vRNA) structures is of high interest. Here, for the first time, we propose a secondary structure for segment 8 vRNA (vRNA8) of A/California/04/2009 (H1N1) formed in the presence of cellular and viral components. This structure shows similarities with prior in vitro experiments. Additionally, we determined the location of several well-defined, conserved structural motifs of vRNA8 within IAV strains with possible functionality. These RNA motifs appear to fold independently of regional nucleoprotein (NP)-binding affinity, but a low or uneven distribution of NP in each motif region is noted. This research also highlights several accessible sites for oligonucleotide tools and small molecules in vRNA8 in a cellular environment that might be a target for influenza A virus inhibition on the RNA level.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Gene Expression Regulation, Viral / Genome, Viral / Influenza A Virus, H1N1 Subtype / Nucleic Acid Conformation Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23052452

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Gene Expression Regulation, Viral / Genome, Viral / Influenza A Virus, H1N1 Subtype / Nucleic Acid Conformation Limits: Animals / Humans Language: English Year: 2022 Document Type: Article Affiliation country: Ijms23052452