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SARS-CoV-2 spike antigen quantification by targeted mass spectrometry of a virus-based vaccine.
Rosen, Osnat; Jayson, Avital; Dor, Eyal; Epstein, Eyal; Makovitzki, Arik; Cherry, Lilach; Lupu, Edith; Monash, Arik; Borni, Sarah; Baruchi, Tzadok; Laskar, Orly; Shmaya, Shlomo; Rosenfeld, Ronit; Levy, Yinon; Schuster, Ofir; Feldberg, Liron.
  • Rosen O; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel. Electronic address: osnatr@iibr.gov.il.
  • Jayson A; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Dor E; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Epstein E; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Makovitzki A; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Cherry L; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Lupu E; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Monash A; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Borni S; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Baruchi T; Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Laskar O; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Shmaya S; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Rosenfeld R; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Levy Y; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel.
  • Schuster O; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel. Electronic address: ofirsc@iibr.gov.il.
  • Feldberg L; Department of Analytical Chemistry, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel. Electronic address: lironf@iibr.gov.il.
J Virol Methods ; 303: 114498, 2022 05.
Article in English | MEDLINE | ID: covidwho-1701734
ABSTRACT
The spike glycoprotein mediates virus binding to the host cells and is a key target for vaccines development. One SARS-CoV-2 vaccine is based on vesicular stomatitis virus (VSV), in which the native surface glycoprotein has been replaced by the SARS-CoV-2 spike protein (VSV-ΔG-spike). The titer of the virus is quantified by the plaque forming unit (PFU) assay, but there is no method for spike protein quantitation as an antigen in a VSV-based vaccine. Here, we describe a mass spectrometric (MS) spike protein quantification method, applied to VSV-ΔG-spike based vaccine. Proof of concept of this method, combining two different sample preparations, is shown for complex matrix samples, produced during the vaccine manufacturing processes. Total spike levels were correlated with results from activity assays, and ranged between 0.3-0.5 µg of spike protein per 107 PFU virus-based vaccine. This method is simple, linear over a wide range, allows quantification of antigen within a sample and can be easily implemented for any vaccine or therapeutic sample.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article