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Progression and Resolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Golden Syrian Hamsters.
Mulka, Kathleen R; Beck, Sarah E; Solis, Clarisse V; Johanson, Andrew L; Queen, Suzanne E; McCarron, Megan E; Richardson, Morgan R; Zhou, Ruifeng; Marinho, Paula; Jedlicka, Anne; Guerrero-Martin, Selena; Shirk, Erin N; Braxton, Alicia M; Brockhurst, Jacqueline; Creisher, Patrick S; Dhakal, Santosh; Brayton, Cory F; Veenhuis, Rebecca T; Metcalf Pate, Kelly A; Karakousis, Petros C; Zahnow, Cynthia A; Klein, Sabra L; Jain, Sanjay K; Tarwater, Patrick M; Pekosz, Andrew S; Villano, Jason S; Mankowski, Joseph L.
  • Mulka KR; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Beck SE; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland; Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Solis CV; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Johanson AL; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Queen SE; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • McCarron ME; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Richardson MR; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Zhou R; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Marinho P; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Jedlicka A; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Guerrero-Martin S; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Shirk EN; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Braxton AM; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Brockhurst J; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Creisher PS; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Dhakal S; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Brayton CF; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Veenhuis RT; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Metcalf Pate KA; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Karakousis PC; Department of Medicine, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Zahnow CA; Department of Oncology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Klein SL; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Jain SK; Department of Pediatrics, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Tarwater PM; Department of Epidemiology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Pekosz AS; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Villano JS; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland.
  • Mankowski JL; Department of Molecular and Comparative Pathobiology, The Johns Hopkins School of Medicine, Baltimore, Maryland; Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, Maryland; Department of Neurology, The Johns Hopkins School of Medicine, Baltimore, Maryland. Electronic address:
Am J Pathol ; 192(2): 195-207, 2022 02.
Article in English | MEDLINE | ID: covidwho-1703223
ABSTRACT
To catalyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research, including development of novel interventive and preventive strategies, the progression of disease was characterized in a robust coronavirus disease 2019 (COVID-19) animal model. In this model, male and female golden Syrian hamsters were inoculated intranasally with SARS-CoV-2 USA-WA1/2020. Groups of inoculated and mock-inoculated uninfected control animals were euthanized at 2, 4, 7, 14, and 28 days after inoculation to track multiple clinical, pathology, virology, and immunology outcomes. SARS-CoV-2-inoculated animals consistently lost body weight during the first week of infection, had higher lung weights at terminal time points, and developed lung consolidation per histopathology and quantitative image analysis measurements. High levels of infectious virus and viral RNA were reliably present in the respiratory tract at days 2 and 4 after inoculation, corresponding with widespread necrosis and inflammation. At day 7, when the presence of infectious virus was rare, interstitial and alveolar macrophage infiltrates and marked reparative epithelial responses (type II hyperplasia) dominated in the lung. These lesions resolved over time, with only residual epithelial repair evident by day 28 after inoculation. The use of quantitative approaches to measure cellular and morphologic alterations in the lung provides valuable outcome measures for developing therapeutic and preventive interventions for COVID-19 using the hamster COVID-19 model.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Animals Language: English Journal: Am J Pathol Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Animals Language: English Journal: Am J Pathol Year: 2022 Document Type: Article