Your browser doesn't support javascript.
CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples.
Aralis, Zach; Rauch, Jennifer N; Audouard, Morgane; Valois, Eric; Lach, Ryan S; Solley, Sabrina; Baxter, Naomi J; Kosik, Kenneth S; Wilson, Maxwell Z; Acosta-Alvear, Diego; Arias, Carolina.
  • Aralis Z; UC Santa Barbara, Santa Barbara, California.
  • Rauch JN; University of Massachusetts Amherst, Amherst, Massachusetts.
  • Audouard M; UC Santa Barbara, Santa Barbara, California.
  • Valois E; UC Santa Barbara, Santa Barbara, California.
  • Lach RS; UC Santa Barbara, Santa Barbara, California.
  • Solley S; UC Santa Barbara, Santa Barbara, California.
  • Baxter NJ; UC Santa Barbara, Santa Barbara, California.
  • Kosik KS; UC Santa Barbara, Santa Barbara, California.
  • Wilson MZ; UC Santa Barbara, Santa Barbara, California.
  • Acosta-Alvear D; UC Santa Barbara, Santa Barbara, California.
  • Arias C; UC Santa Barbara, Santa Barbara, California.
Curr Protoc ; 2(2): e385, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1709165
ABSTRACT
The COVID-19 pandemic has taken a devastating human toll worldwide. The development of impactful guidelines and measures for controlling the COVID-19 pandemic requires continuous and widespread testing of suspected cases and their contacts through accurate, accessible, and reliable methods for SARS-CoV-2 detection. Here we describe a CRISPR-Cas13-based method for the detection of SARS-CoV-2. The assay is called CREST (Cas13-based, rugged, equitable, scalable testing), and is specific, sensitive, and highly accessible. As such, CREST may provide a low-cost and dependable alternative for SARS-CoV-2 surveillance. © 2022 Wiley Periodicals LLC. Basic Protocol Cas13-ased detection of SARS-CoV-2 genetic material using a real-time PCR detection system Alternate Protocol Cas13-based detection of SARS-CoV-2 genetic material using a fluorescence viewer Support Protocol 1 LwaCas13a purification Support Protocol 2 In vitro transcription of synthetic targets.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Curr Protoc Year: 2022 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Curr Protoc Year: 2022 Document Type: Article