CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples.
Curr Protoc
; 2(2): e385, 2022 Feb.
Article
in English
| MEDLINE | ID: covidwho-1709165
ABSTRACT
The COVID-19 pandemic has taken a devastating human toll worldwide. The development of impactful guidelines and measures for controlling the COVID-19 pandemic requires continuous and widespread testing of suspected cases and their contacts through accurate, accessible, and reliable methods for SARS-CoV-2 detection. Here we describe a CRISPR-Cas13-based method for the detection of SARS-CoV-2. The assay is called CREST (Cas13-based, rugged, equitable, scalable testing), and is specific, sensitive, and highly accessible. As such, CREST may provide a low-cost and dependable alternative for SARS-CoV-2 surveillance. © 2022 Wiley Periodicals LLC. Basic Protocol Cas13-ased detection of SARS-CoV-2 genetic material using a real-time PCR detection system Alternate Protocol Cas13-based detection of SARS-CoV-2 genetic material using a fluorescence viewer Support Protocol 1 LwaCas13a purification Support Protocol 2 In vitro transcription of synthetic targets.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Curr Protoc
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS