DNA aptamer selection for SARS-CoV-2 spike glycoprotein detection.
Anal Biochem
; 645: 114633, 2022 05 15.
Article
in English
| MEDLINE | ID: covidwho-1712394
ABSTRACT
The rapid spread of SARS-CoV-2 infection throughout the world led to a global public health and economic crisis triggering an urgent need for the development of low-cost vaccines, therapies and high-throughput detection assays. In this work, we used a combination of Ideal-Filter Capillary Electrophoresis SELEX (IFCE-SELEX), Next Generation Sequencing (NGS) and binding assays to isolate and validate single-stranded DNA aptamers that can specifically recognize the SARS-CoV-2 Spike glycoprotein. Two selected non-competing DNA aptamers, C7 and C9 were successfully used as sensitive and specific biological recognition elements for the development of electrochemical and fluorescent aptasensors for the SARS-CoV-2 Spike glycoprotein with detection limits of 0.07 fM and 41.87 nM, respectively.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Aptamers, Nucleotide
/
COVID-19
Type of study:
Diagnostic study
/
Prognostic study
/
Systematic review/Meta Analysis
Topics:
Vaccines
Limits:
Humans
Language:
English
Journal:
Anal Biochem
Year:
2022
Document Type:
Article
Affiliation country:
J.ab.2022.114633
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