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Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing.
Bloemen, Mandy; Rector, Annabel; Swinnen, Jill; Ranst, Marc Van; Maes, Piet; Vanmechelen, Bert; Wollants, Elke.
  • Bloemen M; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
  • Rector A; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
  • Swinnen J; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
  • Ranst MV; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium; University Hospitals Leuven, Department of Laboratory Medicine, National Reference Center for Respiratory Pathogens, 3000 Leuven, Belgium.
  • Maes P; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
  • Vanmechelen B; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium.
  • Wollants E; KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium. Electronic address: elke.wollants@kuleuven.be.
J Virol Methods ; 304: 114512, 2022 06.
Article in English | MEDLINE | ID: covidwho-1720562
ABSTRACT
SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants. This method can easily distinguish Omicron from other variants.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article Affiliation country: J.jviromet.2022.114512

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article Affiliation country: J.jviromet.2022.114512