Virus-specific editing identification approach reveals the landscape of A-to-I editing and its impacts on SARS-CoV-2 characteristics and evolution.
Nucleic Acids Res
; 50(5): 2509-2521, 2022 03 21.
Article
in English
| MEDLINE | ID: covidwho-1722548
ABSTRACT
Upon SARS-CoV-2 infection, viral intermediates specifically activate the IFN response through MDA5-mediated sensing and accordingly induce ADAR1 p150 expression, which might lead to viral A-to-I RNA editing. Here, we developed an RNA virus-specific editing identification pipeline, surveyed 7622 RNA-seq data from diverse types of samples infected with SARS-CoV-2, and constructed an atlas of A-to-I RNA editing sites in SARS-CoV-2. We found that A-to-I editing was dynamically regulated, varied between tissue and cell types, and was correlated with the intensity of innate immune response. On average, 91 editing events were deposited at viral dsRNA intermediates per sample. Moreover, editing hotspots were observed, including recoding sites in the spike gene that affect viral infectivity and antigenicity. Finally, we provided evidence that RNA editing accelerated SARS-CoV-2 evolution in humans during the epidemic. Our study highlights the ability of SARS-CoV-2 to hijack components of the host antiviral machinery to edit its genome and fuel its evolution, and also provides a framework and resource for studying viral RNA editing.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA Editing
/
SARS-CoV-2
/
COVID-19
/
Immunity, Innate
Type of study:
Experimental Studies
/
Observational study
Limits:
Humans
Language:
English
Journal:
Nucleic Acids Res
Year:
2022
Document Type:
Article
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