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Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples.
Pastorino, Boris; Touret, Franck; Gilles, Magali; Luciani, Lea; de Lamballerie, Xavier; Charrel, Remi N.
  • Pastorino B; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
  • Touret F; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
  • Gilles M; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
  • Luciani L; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
  • de Lamballerie X; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
  • Charrel RN; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
Viruses ; 12(6)2020 06 08.
Article in English | MEDLINE | ID: covidwho-1726020
ABSTRACT
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Type of study: Clinical Practice Guide Limits: Animals / Humans Language: English Year: 2020 Document Type: Article Affiliation country: V12060624

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Virus Inactivation / Betacoronavirus Type of study: Clinical Practice Guide Limits: Animals / Humans Language: English Year: 2020 Document Type: Article Affiliation country: V12060624