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An experimental in silico study on COVID-19: Response of neutrophil-related genes to antibiotics.
Mousavi, Seyyed R; Lotfi, Hajie; Salmanizadeh, Sharareh; Feizbakhshan, Sara; Khosravian, Farinaz; Sajjadi, Maryam S; Komachali, Sajad R; Beni, Faeze A; Torkan, Banafshe; Kazemi, Mohammad; Sami, Ramin; Salehi, Mansoor.
  • Mousavi SR; Cellular, Molecular and Genetics Research Center Isfahan University of Medical Sciences Isfahan Iran.
  • Lotfi H; Medical Genetics Research Center of Genome Isfahan University of Medical Sciences Isfahan Iran.
  • Salmanizadeh S; Cellular and Molecular Research Center Qazvin University of Medical Sciences Qazvin Iran.
  • Feizbakhshan S; Cellular, Molecular and Genetics Research Center Isfahan University of Medical Sciences Isfahan Iran.
  • Khosravian F; Medical Genetics Research Center of Genome Isfahan University of Medical Sciences Isfahan Iran.
  • Sajjadi MS; Cellular, Molecular and Genetics Research Center Isfahan University of Medical Sciences Isfahan Iran.
  • Komachali SR; Medical Genetics Research Center of Genome Isfahan University of Medical Sciences Isfahan Iran.
  • Beni FA; Cellular, Molecular and Genetics Research Center Isfahan University of Medical Sciences Isfahan Iran.
  • Torkan B; Medical Genetics Research Center of Genome Isfahan University of Medical Sciences Isfahan Iran.
  • Kazemi M; Medical Genetics Laboratory Alzahra University Hospital Isfahan University of Medical Sciences Isfahan Iran.
  • Sami R; Cellular, Molecular and Genetics Research Center Isfahan University of Medical Sciences Isfahan Iran.
  • Salehi M; Medical Genetics Research Center of Genome Isfahan University of Medical Sciences Isfahan Iran.
Health Sci Rep ; 5(2): e548, 2022 Mar.
Article in English | MEDLINE | ID: covidwho-1729130
ABSTRACT
Background and

Aims:

All components of the immune system are involved in alleviating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Further research is required to provide detailed insights into COVID-19-related immune compartments and pathways. In addition, a significant percentage of hospitalized COVID-19 patients suspect bacterial infections and antimicrobial resistance occurs following antibiotics treatment. The aim of this study was to evaluate the possible effects of antibiotics on the response of neutrophil-related genes in SARS-CoV-2 patients by an experimental in silico study.

Methods:

The two data sets GSE1739 and GSE21802 including 10 SARS positive patients and 35 influenza A (H1N1) patients were analyzed, respectively. Differentially expressed genes (DEGs) between these two data sets were determined by GEO2R analysis and the Venn diagram online tool. After determining the hub genes involved in immune responses, the expression of these genes in 30 COVID-19 patients and 30 healthy individuals was analyzed by real-time polymerase chain reaction (PCR). All patients received antibiotics, including levofloxacin, colistin, meropenem, and ceftazidime.

Results:

GEO2R analysis detected 240 and 120 DEGs in GSE21802 and GSE1739, respectively. Twenty DEGs were considered as enriched hub genes involved in immune processes such as neutrophil degranulation, neutrophil activation, and antimicrobial humoral response. The central nodes were attributed to the genes of neutrophil elastase (ELANE), arginase 1 (ARG-1), lipocalin 2 (LCN2), and defensin 4 (DEFA4). Compared to the healthy subjects, the expression of LCN2 and DEFA4 were significantly reduced in COVID-19 patients. However, no significant differences were observed in the ELANE and AGR-1 levels between COVID-19 subjects and the control group.

Conclusions:

Activation and degranulation of neutrophils were observed mainly in SARS, and H1N1 infection processes and antibiotics administration could affect neutrophil activity during viral infection. It can be suggested that antibiotics can decrease inflammation by restoring the expression of neutrophil-related genes in COVID-19 patients.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Language: English Journal: Health Sci Rep Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies Language: English Journal: Health Sci Rep Year: 2022 Document Type: Article