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Plasmonic Detection of SARS-CoV-2 Spike Protein with Polymer-Stabilized Glycosylated Gold Nanorods.
Georgiou, Panagiotis G; Guy, Collette S; Hasan, Muhammad; Ahmad, Ashfaq; Richards, Sarah-Jane; Baker, Alexander N; Thakkar, Neer V; Walker, Marc; Pandey, Sarojini; Anderson, Neil R; Grammatopoulos, Dimitris; Gibson, Matthew I.
  • Georgiou PG; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Guy CS; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Hasan M; School of Life Sciences, University of Warwick, CV4 7AL Coventry, U.K.
  • Ahmad A; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Richards SJ; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Baker AN; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Thakkar NV; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Walker M; Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Pandey S; Department of Physics, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
  • Anderson NR; Institute of Precision Diagnostics and Translational Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Clifford Bridge Road Walsgrave, Coventry CV2 2DX, U.K.
  • Grammatopoulos D; Institute of Precision Diagnostics and Translational Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Clifford Bridge Road Walsgrave, Coventry CV2 2DX, U.K.
  • Gibson MI; Warwick Medical School, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.
ACS Macro Lett ; 11(3): 317-322, 2022 03 15.
Article in English | MEDLINE | ID: covidwho-1730252
ABSTRACT
The COVID-19 pandemic has highlighted the need for innovative biosensing, diagnostic, and surveillance platforms. Here we report that glycosylated, polymer-stabilized, gold nanorods can bind the SARS-CoV-2 spike protein and show correlation to the presence of SARS-CoV-2 in primary COVID-19 clinical samples. Telechelic polymers were prepared by reversible addition-fragmentation chain-transfer polymerization, enabling the capture of 2,3-sialyllactose and immobilization onto gold nanorods. Control experiments with a panel of lectins and a galactosamine-terminated polymer confirmed the selective binding. The glycosylated rods were shown to give dose-dependent responses against recombinant truncated SARS-CoV-2 spike protein, and the responses were further correlated using primary patient swab samples. The essentiality of the anisotropic particles for reducing the background interference is demonstrated. This highlights the utility of polymer tethering of glycans for plasmonic biosensors of infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanotubes / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Macro Lett Year: 2022 Document Type: Article Affiliation country: Acsmacrolett.1c00716

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanotubes / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Macro Lett Year: 2022 Document Type: Article Affiliation country: Acsmacrolett.1c00716