Your browser doesn't support javascript.
Comparative Analysis of Results of RT-PCR and Truenat in diagnosis of Covid 19
Indian Journal of Medical Microbiology ; 39:S57-S58, 2021.
Article in English | EMBASE | ID: covidwho-1734465
ABSTRACT

Background:

Almost a year has passed since the start for Covid 19 pandemic, however with no human immunity and no vaccine for prevention early diagnosis remains the mainstay to contain the infection and prevent the spread of the vi- rus. RT-PCR is said to be the most sensitive test currently. However, Truenat is also widely used being approved by ICMR, hence a comparative study of RT-PCR & Truenat was taken up in this study.

Methods:

A total of 200 samples were taken from patients having signs and symptoms (clinically suspected) of COVID 19. Samples obtained via oropharyngeal and nasopharyngeal swabs were analyzed on both RT-PCR and Truenat. Viral load in samples were evaluated using Ct value of targeted genes by both the techniques.

Results:

Out of 200 samples, 184 showed similar results via RT-PCR and Truenat i.e., 61 positive and 123 negatives. 16 samples showed discordant results. Out of 16 samples, 5 were positive and 11 were negative by RT -PCR. However, by Truenat 11 were positive and 5 were negative. The Ct values of targeted genes range between 13-30 for E-gene and 16-32 for RdRp gene.

Conclusions:

The detection of SARS COV-2 patients with mild form of disease (which were persistently negative by RT- PCR) was higher by Truenat. P value being 0.077 which is significant at 90% level of significance. Hence though identifi- cation of viral RNA by RT-PCR is the gold standard, its sensitivity is lower compared to Truenat. Hence, we can suggest that Truenat is a diagnostic method with higher sensitivity, closed system hence lower chances of contamination and at the same time providing faster results at low cost, easy to perform as a point of care test, portable and requiring lower expertise to operate compared to RT-PCR
Keywords

Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Indian Journal of Medical Microbiology Year: 2021 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Indian Journal of Medical Microbiology Year: 2021 Document Type: Article