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Performance of Immunoglobulin G Serology on Finger Prick Capillary Dried Blood Spot Samples to Detect a SARS-CoV-2 Antibody Response.
Nikiforuk, Aidan M; McMillan, Brynn; Bartlett, Sofia R; Márquez, Ana Citlali; Pidduck, Tamara; Kustra, Jesse; Goldfarb, David M; Barakauskas, Vilte; Sinclair, Graham; Patrick, David M; Sadarangani, Manish; Ogilvie, Gina S; Krajden, Mel; Morshed, Muhammad; Sekirov, Inna; Jassem, Agatha N.
  • Nikiforuk AM; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • McMillan B; School of Population and Public Health, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Bartlett SR; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Márquez AC; Department of Experimental Medicine, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Pidduck T; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Kustra J; School of Population and Public Health, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Goldfarb DM; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Barakauskas V; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Sinclair G; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Patrick DM; Department of Pathology and Laboratory Medicine, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Sadarangani M; Department of Pathology and Laboratory Medicine, British Columbia Children's and Women's Hospital, Vancouver, British Columbia, Canada.
  • Ogilvie GS; Department of Pathology and Laboratory Medicine, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Krajden M; Department of Pathology and Laboratory Medicine, British Columbia Children's and Women's Hospital, Vancouver, British Columbia, Canada.
  • Morshed M; Department of Pathology and Laboratory Medicine, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
  • Sekirov I; Department of Pathology and Laboratory Medicine, British Columbia Children's and Women's Hospital, Vancouver, British Columbia, Canada.
  • Jassem AN; British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada.
Microbiol Spectr ; 10(2): e0140521, 2022 04 27.
Article in English | MEDLINE | ID: covidwho-1736038
ABSTRACT
We investigate the diagnostic accuracy and predictive value of finger prick capillary dried blood spot (DBS) samples tested by a quantitative multiplex anti-immunoglobulin G (IgG) assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies after infection or vaccination. This cross-sectional study involved participants (n = 6,841) from several serological surveys conducted in nonhospitalized children and adults throughout 2020 and 2021 in British Columbia (BC), Canada. Analysis used paired DBS and serum samples from a subset of participants (n = 642) prior to vaccination to establish signal thresholds and calculate diagnostic accuracy by logistic regression. Discrimination of the logistic regression model was assessed by receiver operator curve (ROC) analysis in an n = 2,000 bootstrap of the paired sample (n = 642). The model was cross-validated in a subset of vaccinated persons (n = 90). Unpaired DBS samples (n = 6,723) were used to evaluate anti-IgG signal distributions. In comparison to paired serum, DBS samples from an unvaccinated population possessed a sensitivity of 79% (95% confidence interval [95% CI] 58 to 91%) and specificity of 97% (95% CI 95 to 98%). ROC analysis found that DBS samples accurately classify SARS-CoV-2 seroconversion at an 88% percent rate (area under the curve [AUC] = 88% [95% CI 80 to 95%]). In coronavirus disease 2019 (COVID-19) vaccine dose one or two recipients, the sensitivity of DBS testing increased to 97% (95% CI 83 to 99%) and 100% (95% CI 88 to 100%). Modeling found that DBS testing possesses a high positive predictive value (98% [95% CI 97 to 98%]) in a population with 75% seroprevalence. We demonstrate that DBS testing should be considered to reliably detect SARS-CoV-2 seropositivity from natural infection or vaccination. IMPORTANCE Dried blood spot samples have comparable diagnostic accuracy to serum collected by venipuncture when tested by an electrochemiluminescent assay for antibodies and should be considered to reliably detect seropositivity following SARS-CoV-2 infection and/or vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Adult / Child / Humans Language: English Journal: Microbiol Spectr Year: 2022 Document Type: Article Affiliation country: Spectrum.01405-21

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Adult / Child / Humans Language: English Journal: Microbiol Spectr Year: 2022 Document Type: Article Affiliation country: Spectrum.01405-21