Your browser doesn't support javascript.
Novel T cell interferon gamma release assay (IGRA) using spike recombinant protein for COVID19 vaccine response and Nucleocapsid for SARS-Cov2 response.
Renaudineau, Yves; Abravanel, Florence; Izopet, Jacques; Bost, Chloé; Treiner, Emmanuel; Congy, Nicolas; Blancher, Antoine.
  • Renaudineau Y; Immunology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France. Electronic address: renaudineau.y@chu-toulouse.fr
  • Abravanel F; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France; Virology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France. Electronic address: abravanel.f@chu-toulouse.fr.
  • Izopet J; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France; Virology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France. Electronic address: izopet.j@chu-toulouse.fr.
  • Bost C; Immunology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France. Electronic address: bost.c@chu-toulouse.fr.
  • Treiner E; Immunology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France. Electronic address: treiner.e@chu-toulouse.fr.
  • Congy N; Immunology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France. Electronic address: congy.n@chu-toulouse.fr.
  • Blancher A; Immunology department laboratory, Institut Fédératif de Biologie, Toulouse University Hospital Center, France; INFINITy, Toulouse Institute for Infectious and Inflammatory Diseases, INSERM U1291, CNRS U5051, University Toulouse III, Toulouse, France. Electronic address: blancher.antoine@neuf.fr.
Clin Immunol ; 237: 108979, 2022 04.
Article in English | MEDLINE | ID: covidwho-1739616
ABSTRACT
We explored the performance of a whole blood interferon gamma release assay (IGRA) based on the stimulation of SARS-Cov2-specific T cells by purified recombinant proteins. Twenty volunteers vaccinated with BNT162b2 were selected first for T cell response evaluation using an in-house IGRA, a commercial IGRA, and ELISpot showing a S2 > S1 poly-epitopic response. Next, 64 vaccinated and 103 non-vaccinated individuals were tested for humoral and T cell response (IGRA-Spike/-nucleocapsid recombinant proteins). Following the second vaccine injection, humoral (100%) and IGRA-Spike T cell (95.3%) responses took place irrespective of sex, age, and vaccine type. The humoral response declined first, followed by IGRA-Spike T cell response after the second vaccine injection. Altogether, this study confirms the utility of the IGRA-Spike/-nucleocapsid assay to complement serology in COVID19 vaccinated individuals and those who have recovered from SARS-Cov2.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon-gamma Release Tests / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Clin Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon-gamma Release Tests / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Clin Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article