Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples.
STAR Protoc
; 3(1): 101203, 2022 03 18.
Article
in English
| MEDLINE | ID: covidwho-1740310
ABSTRACT
Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Immunoassay
/
Spike Glycoprotein, Coronavirus
/
SARS-CoV-2
/
Milk, Human
/
Antibodies, Viral
Type of study:
Qualitative research
Topics:
Vaccines
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2022
Document Type:
Article
Affiliation country:
J.xpro.2022.101203
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